EurekaMag.com logo
+ Translate

2-Oxoglutarate-dependent dioxygenase and related enzymes: biochemical characterization


, : 2-Oxoglutarate-dependent dioxygenase and related enzymes: biochemical characterization. Phytochemistry 36(5): 1093-1107

Hydroxylation reactions are catalysed by a few major subclasses of enzymes which are ubiquitously distributed in nature. Dioxygenases generally occur as soluble enzymes where they catalyse a diversity of oxygenation reactions in a large number of metabolic pathways in animals, plants and micro-organisms. This review discusses recent advances in the biochemistry and molecular biology of dioxygenases occurring in different biological systems.

Accession: 002549869

PMID: 7765359

DOI: 10.1016/s0031-9422(00)89621-1

Download PDF Full Text: 2-Oxoglutarate-dependent dioxygenase and related enzymes: biochemical characterization


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

D.C.rolis, E.; D.L.ca, V., 1994: 2-oxoglutarate-dependent dioxygenase and related enzymes: biochemical characterization. Hydroxylation reactions are catalysed by a few major subclasses of enzymes which are ubiquitously distributed in nature. Dioxygenases generally occur as soluble enzymes where they catalyse a diversity of oxygenation reactions in a large number of...

D.C.rolis, E.; Chan, F.; Balsevich, J.; D.L.ca, V., 1990: Isolation and Characterization of a 2-Oxoglutarate Dependent Dioxygenase Involved in the Second-to-Last Step in Vindoline Biosynthesis. Young leaves from Catharanthus roseus plants contain the enzymes which convert the monoterpenoid indole alkaloid, tabersonine by three hydroxylations, two methylations, and one acetylation step to vindoline. A novel direct enzyme assay has been de...

Anzellotti, D.; Ibrahim, R.K., 2000: Novel flavonol 2-oxoglutarate dependent dioxygenase: affinity purification, characterization, and kinetic properties. A 2-oxoglutarate-dependent dioxygenase (EC 1.14.11-) that catalyzes the 6-hydroxylation of partially methylated flavonols has been purified to near homogeneity from Chrysosplenium americanum. Enzyme purification was achieved by fast protein liquid...

White, A.K.; Metcalf, W.W., 2002: Isolation and biochemical characterization of hypophosphite/2-oxoglutarate dioxygenase. A novel phosphorus-oxidizing enzyme from Psuedomonas stutzeri WM88. The htxA gene is required for the oxidation of hypophosphite in Pseudomonas stutzeri WM88 (Metcalf, W. W., and Wolfe, R. S. (1998) J. Bacteriol. 180, 5547-5558). Amino acid sequence comparisons suggest that hypophosphite:2-oxoglutarate dioxygenase...

White, A.K.; Metcalf, W.W., 2002: Isolation and biochemical characterization of hypophosphite/2-oxoglutarate dioxygenase. A novel phosphorus-oxidizing enzyme from Pseudomonas stutzeri WM88. The htxA gene is required for the oxidation of hypophosphite in Pseudomonas stutzeri WM88 (Metcalf, W. W., and Wolfe, R. S. (1998) J. Bacteriol. 180, 5547-5558). Amino acid sequence comparisons suggest that hypophosphite:2-oxoglutarate dioxygenase...

De-Carolis, E.; De-Luca, V., 1994: A novel 2-oxoglutarate-dependent dioxygenase involved in vindoline biosynthesis: Characterization, purification and kinetic properties. The enzyme, desacetoxyvindoline 4-hydroxylase, was purified to apparent homogeneity from Catharanthus roseus by ammonium sulfate precipitation and successive chromatography on Sephadex G-100, green 19-agarose, hydroxylapatite, alpha-kg sepharose a...

D.C.rolis, E.; D.L.ca, V., 1992: Purification and characterization of a 2-oxoglutarate-dependent dioxygenase involved in vindoline biosynthesis from Catharanthus roseus. Singh, B K [Editor], Flores, H E [Editor], Shannon, J C [Editor] Current Topics in Plant Physiology an American Society of Plant Physiologists Series; Biosynthesis and molecular regulation of amino acids in plants : 367-368

Bhattacharya, A.; Kourmpetli, S.; Ward, D.A.; Thomas, S.G.; Gong, F.; Powers, S.J.; Carrera, E.; Taylor, B.; de Caceres Gonzalez, F.Nuñez.; Tudzynski, B.; Phillips, A.L.; Davey, M.R.; Hedden, P., 2013: Characterization of the fungal gibberellin desaturase as a 2-oxoglutarate-dependent dioxygenase and its utilization for enhancing plant growth. The biosynthesis of gibberellic acid (GA<SUB>3</SUB>) by the fungus Fusarium fujikuroi is catalyzed by seven enzymes encoded in a gene cluster. While four of these enzymes are characterized as cytochrome P450 monooxygenases, the nature...

D.C.rolis, E.; D.L.ca, V., 1993: Purification, characterization, and kinetic analysis of a 2-oxoglutarate-dependent dioxygenase involved in vindoline biosynthesis from Catharanthus roseus. A 2-oxoglutarate-dependent dioxygenase (EC 1.14.11.11) which catalyzes the hydroxylation at position 4 of the indole alkaloid, desacetoxyvindoline has been purified to near homogeneity from Catharanthus roseus. The purification procedure combined...

Vazquez Flota, F.; Carolis, E. de; Alarco, A.M.; Luca, V. de, 1997: Molecular cloning and characterization of desacetoxyvindoline-4-hydroxylase, a 2-oxoglutarate dependent-dioxygenase involved in the biosynthesis of vindoline in Catharanthus roseus (L.) G. Don. A 2-oxoglutarate-dependent dioxygenase (EC 1.14.11.11) which catalyzes the 4-hydroxylation of desacetoxyvindoline was purified to homogeneity. Three oligopeptides isolated from a tryptic digest of the purified protein were microsequenced and one o...