A study on the practicality of monoclonal antibodies to a densonucleosis virus of the silkworm (Bombyx mori)

Chen, J.G.; Hu, C.

Acta Agriculturae Universitatis Zhejiangensis 18(1): 7-10


Accession: 002556267

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A diagnostic kit was developed to detect a densonucleosis virus of Bombyx mori, using monoclonal antibodies and the double immunodiffusion technique. Using the kit, the virus coudl be diagnosed 48 h after infection. When both monoclonal antibodies and immuno-enzymes or immunofluorescence were used the virus antigen could be detected in the midgut in 12-16 h after infection. The titre of the antibody liquid in the kit was stable, and the liquid was still effective after 1 month at 37 degrees C or 2 months at room temperature (23 degrees C).