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Accuracy of most-probable-number estimates of rhizobia for tree legumes



Accuracy of most-probable-number estimates of rhizobia for tree legumes



Soil Biology & Biochemistry 25(1): 69-74



Rhizobia that nodulate tree legumes have rarely been enumerated by the most-probable-number (MPN) plant infection assay. We compared MPN estimates of pure cultures of rhizobia with plate counts, using as hosts seedlings of 14 tree legume species grown separately in growth pouches or glass tubes. Reasonable agreement was obtained with 11 of the 14 tree species in one or both growth systems, with closer agreement in glass tubes than growth pouches for small-seeded species. Weekly assessment of MPN assays indicated that glass tubes and growth pouches should be scored for nodulation at least 7 and 5 weeks after inoculation, respectively.

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Accession: 002557704

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DOI: 10.1016/0038-0717(93)90243-5



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