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An improved colorimetric method for the estimation of lactulose in lactose-lactulose mixtures



An improved colorimetric method for the estimation of lactulose in lactose-lactulose mixtures



Food chemistry 3(5): 399-402



An improved colorimetric method is described for the estimation of lactulose in mixtures of lactose and lactulose. The anthrone method for the estimation of lactulose was modified by altering the concentrations of alcohol and acetic acid added to the reaction mixture. This resulted in a two-fold increase in the sensitivity of the method and also facilitated increase in sample volume. Values for lactulose estimated in lactose-lactulose mixtures using this method, compared well with those obtained using a chromatographic method involving a prior separation of lactulose.

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Accession: 002562524

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DOI: 10.1016/0308-8146(92)90314-r


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