+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Baculovirus p35 prevents developmentally programmed cell death and rescues a ced-9 mutant in the nematode Caenorhabditis elegans



Baculovirus p35 prevents developmentally programmed cell death and rescues a ced-9 mutant in the nematode Caenorhabditis elegans



Embo Journal 13(9): 2023-2028



Programmed cell death, or apoptosis, occurs throughout the course of normal development in most animals and can also be elicited by a number of stimuli such as growth factor deprivation and viral infection. Certain morphological and biochemical characteristics of programmed cell death are similar among different tissues and species. During development of the nematode Caenorhabditis elegans, a single genetic pathway promotes the death of selected cells in a lineally fixed pattern. This pathway appears to be conserved among animal species. The baculovirus p35-encoding gene (p35) is an inhibitor of virus-induced apoptosis in insect cells. Here we demonstrate that expression of p35 in C. elegans prevents death of cells normally programmed to die. This suppression of developmentally programmed cell death results in appearance of extra surviving cells. Expression of p35 can rescue the embryonic lethality of a mutation in ced-9, an endogenous gene homologous to the mammalian apoptotic suppressor bcl-2, whose absence leads to ectopic cell deaths. These results support the hypothesis that viral infection can activate the same cell death pathway as is used during normal development and suggest that baculovirus p35 may act downstream or independently of ced-9 in this pathway.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002568761

Download citation: RISBibTeXText

PMID: 8187756

DOI: 10.1002/j.1460-2075.1994.tb06475.x


Related references

Non-developmentally programmed cell death in Caenorhabditis elegans. Seminars in Cancer Biology 17(2): 122-133, 2007

BCL-2 prevents programmed cell death in Caenorhabditis elegans. Journal of Cellular Biochemistry Supplement 0(17 PART B): 191, 1993

The genetics of programmed cell death in the nematode Caenorhabditis elegans. Cold Spring Harbor Symposia on Quantitative Biology 59: 377-385, 1994

Analysis of programmed cell death in the nematode Caenorhabditis elegans. Methods in Enzymology 322: 76-88, 2000

Genetic control of programmed cell death in the nematode Caenorhabditis elegans. Cancer Research 59(7 Suppl): 1701s-1706s, 1999

Genetic control of programmed cell death in the nematode Caenorhabditis elegans. Molecular Biology of the Cell 5(SUPPL ): 251A, 1994

Genetic control of programmed cell death and aging in the nematode Caenorhabditis elegans. Experimental Gerontology. 32(4-5): 363-374, 1997

Mutations affecting programmed cell deaths in the nematode Caenorhabditis elegans. Science 220(4603): 1277-1279, 1983

Programmed cell death in Caenorhabditis elegans. Current Opinion in Genetics & Development 4(4): 581-586, 1994

Life and death decisions: ced-9 and programmed cell death in Caenorhabditis elegans. Science Washington 270(5238): 931, 1995

The Caenorhabditis elegans cell death gene ced-4 encodes a novel protein and is expressed during the period of extensive programmed cell death. Development 116(2): 309-320, 1992

Programmed and pathological cell death in Caenorhabditis elegans. Holbrook, N J, Martin, G R, Lockshin, R A Modern Cell Biology; Cellular aging and cell death 235-253, 1996

Programmed Cell Death During Caenorhabditis elegans Development. Genetics 203(4): 1533-1562, 2017

The engulfment process of programmed cell death in caenorhabditis elegans. Annual Review of Cell and Developmental Biology 20: 193-221, 2004

Mechanisms of programmed cell death in Caenorhabditis elegans and vertebrates. Current Topics in Developmental Biology 32: 139-174, 1996