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Bioassay for evaluation of the pathogenicity of Beauveria bassiana (Bals.) Vuill. to the coffee borer, Hypothenemus hampei (Ferrari)



Bioassay for evaluation of the pathogenicity of Beauveria bassiana (Bals.) Vuill. to the coffee borer, Hypothenemus hampei (Ferrari)



Revista Colombiana de Entomologia 19(4): 123-130



A bioassay was developed to test the pathogenicity of Beauveria bassiana to Hypothenemus hampei. Adults were exposed to the fungus at 1 x 107 conidia/ml for 2 min and then transferred to individual glass vials. The activity of infected insects was reduced, they lost legs and antennae and were unable to bore into coffee berries. Mortality was 88.88% for the fungus isolate Bb-9205 DS and 100% with Bb-9205 BFC, with respective LT50s of 92.4 and 54.72 h.

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