EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Characterization of pectate lyase produced by Pseudomonas marginalis and cloning of pectate lyase genes


Characterization of pectate lyase produced by Pseudomonas marginalis and cloning of pectate lyase genes



Physiological and Molecular Plant Pathology 46(2): 109-119



ISSN/ISBN: 0885-5765

DOI: 10.1006/pmpp.1995.1009

Pseudomonas marginalis produced two pectate lyase isozymes in a medium containing sodium polypectate. Of the two, one had alkaline pI, 9.5, and the other had neutral pI, 7.0. Extracellular pectate lyase secretion started during mid-log phase and reached a maximum during the stationary phase of growth. Pectate lyase production was improved by the addition of 1 mM CaCl-2 which also enhanced the extracellular secretion in the medium containing glucose as carbon source. Ca-2+ facilitated the utilization of polygalacturonic acid as carbon source. Pectate lyase (pel) genes were cloned in pUC18 vector. One pel clone (pME1) containing a 7.7 kb BamHI fragment coded for both alkaline and neutral isozymes. A restriction map was constructed for the plasmid pME1. pel genes were sub-cloned from plasmid pME1. Sub-cloning and restriction mapping indicated that a 2.3 kb PstI-BamH1 fragment coded for one isozyme and a 2.8 kb Pst1 fragment coded for another isozyme; these two genes were separated by 2.6 kb. Southern hybridization analysis indicated that P. marginalis pel genes are homologous with Erwinia pel genes. Escherichia coli containing pel genes efficiently macerated potato tuber tissue.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002577419

Download citation: RISBibTeXText

Related references

Molecular cloning and nucleotide sequence of the pectate lyase gene from Pseudomonas marginalis N6301. Bioscience Biotechnology and Biochemistry 57(6): 957-960, 1993

Screening of a Novel Polysaccharide Lyase Family 10 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression and Characterization. Molecules 23(11), 2018

Improving the Catalytic Performance of Pectate Lyase Through Pectate Lyase/Cu 3 (PO 4 ) 2 Hybrid Nanoflowers as an Immobilized Enzyme. Frontiers in Bioengineering and Biotechnology 8: 280, 2020

Cloning and characterization of a pectate lyase gene from Pseudomonas syringae pv lachrymans. Phytopathology 85(10): 1159, 1995

Molecular cloning and characterization of the pectate lyase gene of pseudomonas fluorescens. Abstracts of the Annual Meeting of the American Society for Microbiology 89: 296, 1989

Regulation of extracellular pectate lyase in erwinia chrysanthemi evidence that reaction products of pectate lyase and exo poly alpha d galacturonosidase mediate induction on d galacturonan. Physiological Plant Pathology 21(2): 127-140, 1982

Regulation of extracellular pectate lyase in Erwinia chrysanthemi: evidence that reaction products of pectate lyase and exo-poly-alpha-D-galacturonosidase mediate induction on D-galacturonan in the soft rot diseases. Physiological plant pathology 21(2): 127-139, 1982

Cloning and characterization of a pectate lyase gene from the soft-rotting bacterium Pseudomonas viridiflava. Molecular Plant-Microbe Interactions: Mpmi 5(4): 301-308, 1992

Use of Tn5tac1 to clone a pel gene encoding a highly alkaline, asparagine-rich pectate lyase isozyme from an Erwinia chrysanthemi EC16 mutant with deletions affecting the major pectate lyase isozymes. Journal of Bacteriology 177(15): 4553-4556, 1995

Identification of gene loci controlling pectate lyase production and soft-rot pathogenicity in Pseudomonas marginalis. Canadian Journal of Microbiology 43(5): 425-431, 1997

Cloning of a pectate lyase gene from Xanthomonas campestris pv. malvacearum and comparison of its sequence relationship with pel genes of soft-rot Erwinia and Pseudomonas. Molecular Plant-Microbe Interactions: Mpmi 9(1): 14-21, 1996

Cloning and characterization of two ripening-related strawberry (Fragaria x ananassa cv. Chandler) pectate lyase genes. Journal of Experimental Botany 54(383): 633-645, 2003

Cloning of the erwinia chrysanthemi strain ec 16 pel genes biochemical and immunological characterization of the pectate lyase isozymes. Abstracts of the Annual Meeting of the American Society for Microbiology 86: 212, 1986

Cloning of two pectate lyase genes from the marine Antarctic bacterium Pseudoalteromonas haloplanktis strain ANT/505 and characterization of the enzymes. Extremophiles: Life Under Extreme Conditions 5(1): 35-44, 2001

Resolution of four pectate lyase structural genes of Erwinia chrysanthemi(Ec16) and characterization of the enzymes produced in Escherichia coII. Molecular Genetics and Genomics 209(2): 319-325, 1987