Chromosomal behavior of the somatic hybrids derived from Nicotiana sylvestris and N. tomentosiformis protoplast fusion

Liao, L.J.; Lai, K.L.

Special Publication Taichung District Agricultural Improvement Station 35: 215-221


Accession: 002579065

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Root tips of 43 hybrid clones were stained with Feulgen solution and examined by light microscopy. The chromosome number ranged from 42 to 68, with 30 clones having 2n = 48 (N. sylvestris and N. tomentosiformis both have 2n = 24). Somatic hybrids with 48 chromosomes had various aberrations in characters such as leaf shape, flower colour and peroxidase isoenzyme pattern. Results indicate that the cytoplasmic constitution may be different among the derived somatic hybrids. Most of the homologous chromosomes paired normally at diakinesis in PMCs, but fertility of the somatic hybrid plants was extremely low. The morphological traits of plants with chromosome numbers other than 2n = 48 were quite different from those of plants with 2n = 48, indicating the possibility of aneuploidy.