+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Concentration of fish enveloped viruses from large volumes of water

Concentration of fish enveloped viruses from large volumes of water

Journal of Virological Methods 43(1): 31-40

ISSN/ISBN: 0166-0934

PMID: 8360314

DOI: 10.1016/0166-0934(93)90087-8

The validity of several concentration procedures for the detection of fish enveloped viruses present in large volumes of water was determined. Viral haemorrhagic septicaemia virus (VHSV) was used to evaluate adsorption/elution to positively-charged MK filter cartridges for the concentration of enveloped viruses. For fresh water, the efficiency of the procedure ranged from 12 to 100%, with a mean recovery of 57%. In seawater samples, the recoveries varied from 15 to 100%, with a mean recovery of 59%. The same virus was used in methods such as organic flocculation and ammonium sulphate flocculation with very poor recoveries of infectious virus, caused by the inactivation of VHSV in both procedures. Concentration of seawater samples from tanks housing sea bass or gilthead affected by viral erythrocytic infection and lymphocystis, respectively, were carried out. In both cases, the viruses responsible for the outbreaks were detected by electron microscopy in the concentrated water samples.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 002584404

Download citation: RISBibTeXText

Related references

Concentration of viruses from large volumes of tap water using pleated membrane filters. Applied and Environmental Microbiology 31(2): 221-226, 1976

Simple field method for concentration of viruses from large volumes of water. Applied and Environmental Microbiology 48(2): 431-432, 1984

Development and validation of a concentration method for the detection of influenza a viruses from large volumes of surface water. Applied and Environmental Microbiology 77(11): 3802-3808, 2011

A simple method for the detection of low concentration of viruses in large volumes of water by the membrane filter technique. Canadian Journal of Microbiology 15(5): 399-403, 1969

Concentration of enteric viruses in large volumes of water using a cartridge-type mixed cellulose ester membrane. Food and Environmental Virology 7(1): 7-13, 2015

Glass Wool Concentration Optimization for the Detection of Enveloped and Non-enveloped Waterborne Viruses. Food and Environmental Virology 11(2): 184-192, 2019

Concentration of Giardia lamblia cysts, Legionella pneumophila, Clostridium perfringens, human enteric viruses, and coliphages from large volumes of drinking water, using a single filtration. Canadian Journal of Microbiology 35(10): 932-935, 1989

Problems related to the concentration of viruses from large volumes of wastewater. Virus Aspects of Applying Municipal Waste to Land Symposium Proceedings: 7-44, 1976

Concentration method for the detection of enteric viruses from large volumes of foods. Journal of Food Protection 72(9): 2001-2005, 2009

Deciphering the Origin and Evolution of Hepatitis B Viruses by Means of a Family of Non-enveloped Fish Viruses. Cell Host and Microbe 22(3): 387-399.E6, 2017

A portable device for the rapid concentration of viruses from large volumes of natural freshwater. Journal of Virological Methods 3(4): 241-249, 1981

Detection of viruses in large volumes of natural waters by concentration on insoluble polyelectrolytes. Water Research 4(12): 787-796, 1970

Concentration of oncornavirus and herpes type viruses from large volumes of tissue culture fluids by molecular filtration. Abstracts of the Annual Meeting of the American Society for Microbiology 75: 271, 1975

Developments in methods for the detection of viruses in large volumes of water. Water Science and Technology 15(5): 59-68, 1983

Efficiency of ultra filtration for the isolation of enteric viruses and coli phages from large volumes of water in studies on waste water reclamation. Water Science and Technology 13(2): TOR 851-TOR 864, 1981