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Degradation and utilization of forage hemicellulose by rumen bacteria, singly in coculture or added sequentially


, : Degradation and utilization of forage hemicellulose by rumen bacteria, singly in coculture or added sequentially. Journal of Applied Bacteriology 77(5): 541-548

Procedures for sequential addition experiments were developed to study the mechanisms involved in the synergistic and inhibitory interactions observed in forage hemicellulose digestion by rumen bacterial cocultures. One organism was allowed to ferment a forage substrate, the culture tube was sterilized and then inoculated with a second organism. No differences were found in the extent of degradation or utilization between fermentations sterilized by oxidation or heat, and based on ease of handling, heat was used in all subsequent experiments. Studies were conducted with Fibrobacter succinogenes A3c, Ruminococcus flavefaciens B34b and Prevotella ruminicola H2b, singly and in all possible combinations. Results from the sequential addition studies substantiated earlier suggestions that the increase observed in hemicellulose utilization results from initial solubilization of the hemicellulose from the forage by the non-utilizer and subsequent utilization of this solubilized polysaccharide by the utilizing, but non-degrading organism.

Accession: 002590001

PMID: 8002478

DOI: 10.1111/j.1365-2672.1994.tb04399.x

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Related references

Osborne J.M.; Dehority B.A., 1989: Synergism in the degradation and utilization of intact forage cellulose hemicellulose and pectin by three pure cultures of rumen bacteria. Journal of Animal Science 67(SUPPL 2): 150

Coen, J.A.; Dehority, B.A., 1970: Degradation and utilization of hemicellulose from intact forages by pure cultures of rumen bacteria. Several pure strains of rumen bacteria have previously been shown to degrade isolated hemicelluloses from a form insoluble in 80% acidified ethanol to a soluble form, regardless of the eventual ability of the organism to utilize the end products a...

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