EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Degradation of 4,4'-dichlorobiphenyl, 3,3',4,4'-tetrachlorobiphenyl, and 2,2',4,4',5,5'-hexachlorobiphenyl by the white rot fungus Phanerochaete chrysosporium


Applied and Environmental Microbiology 61(11): 3904-3909
Degradation of 4,4'-dichlorobiphenyl, 3,3',4,4'-tetrachlorobiphenyl, and 2,2',4,4',5,5'-hexachlorobiphenyl by the white rot fungus Phanerochaete chrysosporium
The white rot fungus Phanerochaete chrysosporium has demonstrated abilities to degrade many xenobiotic chemicals. In this study, the degradation of three model polychlorinated biphenyl (PCB) congeners (4,4'-dichlorobiphenyl [DCB], 3,3',4,4'-tetrachlorobiphenyl, and 2,2',4,4',5,5'-hexachlorobiphenyl) by P. chrysosporium in liquid culture was examined. After 28 days of incubation, 14C partitioning analysis indicated extensive degradation of DCB, including 11% mineralization. In contrast, there was negligible mineralization of the tetrachloro- or hexachlorobiphenyl and little evidence for any significant metabolism. With all of the model PCBs, a large fraction of the 14C was determined to be biomass bound. Results from a time course study done with 4,4'-[14C]DCB to examine 14C partitioning dynamics indicated that the biomass-bound 14C was likely attributable to nonspecific adsorption of the PCBs to the fungal hyphae. In a subsequent isotope trapping experiment, 4-chlorobenzoic acid and 4-chlorobenzyl alcohol were identified as metabolites produced from 4,4'-[l4C]DCB. To the best of our knowledge, this the first report describing intermediates formed by P. chrysosporium during PCB degradation. Results from these experiments suggested similarities between P. chrysosporium and bacterial systems in terms of effects of congener chlorination degree and pattern on PCB metabolism and intermediates characteristic of the PCB degradation process.


Accession: 002590022

PMID: 8526503



Related references

Metabolism of 4,4'-dichlorobiphenyl by white-rot fungi Phanerochaete chrysosporium and Phanerochaete sp. MZ142. Applied Microbiology and Biotechnology 72(3): 566-575, 2006

Degradation of chlorobenzenes by the white-rot fungus Phanerochaete chrysosporium. Abstracts of the General Meeting of the American Society for Microbiology 94(0): 466, 1994

Degradation of cyanides by the white rot fungus Phanerochaete chrysosporium. ACS Symposium series American Chemical Society: 18) 191-202, 1993

Degradation of isoproturon by the white rot fungus Phanerochaete chrysosporium. Biology and fertility of soils 33(6): 521-528, 2001

Degradation of petroleum oil by white rot fungus Phanerochaete chrysosporium. Pages 1035-1038 1996, 1996

Degradation of hazardous organics by the white rot fungus phanerochaete chrysosporium. Abstracts Of Papers American Chemical Society: Nvr 217, 1987

Initial Steps in the Degradation of Methoxychlor by the White Rot Fungus Phanerochaete chrysosporium. Applied and Environmental Microbiology 63(3): 1175-1177, 1997

Decolorization and lignin degradation by immobilized white rot fungus Phanerochaete chrysosporium. Revue Roumaine de Biochimie 31(2-3): 133-138, 1994

Degradation of 4-chlorophenol by the white rot fungus Phanerochaete chrysosporium in free and immobilized cultures. Bioresource Technology 84(2): 145-150, 2002

Effect of nutrient nitrogen on the degradation of pentachlorophenol by white rot fungus, Phanerochaete chrysosporium. Journal of Microbiology & Biotechnology 11(4): 704-708, August, 2001