+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Deletion of the last two exons of the mitochondrial nad7 gene results in lack of the NAD7 polypeptide in a Nicotiana sylvestris CMS mutant

, : Deletion of the last two exons of the mitochondrial nad7 gene results in lack of the NAD7 polypeptide in a Nicotiana sylvestris CMS mutant. Molecular & General Genetics 248(1): 79-88

In Nicotiana sylvestris, two cytoplasmic male sterile (CMS) mutants obtained by protoplast culture show abnormal developmental features of both vegetative and reproductive organs, and mitochondrial gene reorganization following homologous recombination between 65 bp repeated sequences. A mitochondrial region of 16.2 kb deleted from both CMS mutants was found to contain the last two exons of the nad7 gene coding for a subunit of the mitochondrial respiratory chain complex I, which is encoded in the nucleus in fungi and animals but was recently found to be encoded by the mitochondrial genome in wheat. Although the N. sylvestris nad 7 gene shows strong homology with its wheat counterpart, it contains only three introns instead of four. Polymerase chain reaction (PCR) experiments indicated that the parental gene organization, including the complete nad7 gene, is probably maintained at a substoichiometric level in the CMS mutants, but this proportion is too low to have a significant physiological role, as confirmed by expression studies showing the lack of detectable amounts of the NAD7 polypeptide. Consequently, absence of NAD7 is not lethal to plant cells but a deficiency of complex I could be involved in the abnormal CMS phenotype.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002590217

PMID: 7651330

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Borst, P.; Grivell, L.A., 1981: Small is beautiful--portrait of a mitochondrial genome. Nature 290(5806): 443-444

Tantsev, D.; Ivanov, S., 1995: A comparison of grain sorghum and maize grown under irrigation. Sorghum hybrid Fundulea 21 and maize hybrid Knezha 61 were grown in 1987-89 in cinnamon forest soil and given 160 kg N, 100 kg P and 60 kg K/ha. Crops were irrigated with 700 msuperscript 3 water/ha to give a soil water content of 75-80% on 0-4 oc...

Choi, S-Ran.; Kwon, S-Gu.; Choi, H-Seong.; Han, H-Jae.; Beitz, A.James.; Lee, J-Hern., 2016: Neuronal NOS Activates Spinal NADPH Oxidase 2 Contributing to Central Sigma-1 Receptor-Induced Pain Hypersensitivity in Mice. NlmCategory="UNASSIGNED">We recently demonstrated that activation of spinal sigma-1 receptors (Sig-1Rs) induces pain hypersensitivity via the activation of neuronal nitric oxide synthase (nNOS) and nicotinamide adenine dinucleotide ph...

MIDDLETON, J.M., 1947: Selenium burn of the eye; report of a case, with review of the literature. Archives of Ophthalmology 38(6): 806-811

Turcotte, E.; Wiens, L.W.; Grierson, J.R.; Peterson, L.M.; Wener, M.H.; Vesselle, H., 2007: Toxicology evaluation of radiotracer doses of 3'-deoxy-3'-[18F]fluorothymidine (18F-FLT) for human PET imaging: Laboratory analysis of serial blood samples and comparison to previously investigated therapeutic FLT doses. 18F-FLT is a novel PET radiotracer which has demonstrated a strong potential utility for imaging cellular proliferation in human tumors in vivo. To facilitate future regulatory approval of 18F-FLT for clinical use, we wished to demonstrate the saf...

Enhörning, G., 1969: Expansion of the lungs in the new-born and its effect on pulmonary circulation. Acta Obstetricia et Gynecologica Scandinavica 48: Suppl 3:72+-Suppl 3:72+

Maksymowych, W.P.; Ikawa, T.; Yamaguchi, A.; Ikeda, M.; McDonald, D.; Laouar, L.; Lahesmaa, R.; Tamura, N.; Khuong, A.; Yu, D.T.; Kane, K.P., 1998: Invasion by Salmonella typhimurium induces increased expression of the LMP, MECL, and PA28 proteasome genes and changes in the peptide repertoire of HLA-B27. We have analyzed proteasomal adaptation and associated changes in the B27-bound peptide repertoire in response to cellular invasion with Salmonella. The peptide repertoire of HLA-B27 complexes was analyzed by two different methods: (i) high-pressu...

Petrović, Z.; Krstić, L., 1981: Surgery of an eye injury caused by a fishing hook. Vojnosanitetski Pregled 38(4): 267-269

Ostrolenk, M.; Hunter, A.C., 1939: Bacteria of the Colon-Aerogenes group on nut meats. No coliform bacteria were demonstrated on any of 34 samples of nuts in the shell, the samples having been shelled aseptically. Escherichia coli was present in all but 2 of 11 vars. in from 4 to 68% of 548 samples representing 200 retail market sam...

Svrcek, M.M.theis, W., 1979: Finding of Helotium longisporum Schweers in Switzerland and its identity with Phaeohelotium geogenum (Cooke) comb. nov. Ceska mykologie 33(4): 225-228