EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Delivery of a live calf after in vitro maturation and fertilization of oocytes obtained from ovaries removed from a moribund cow






Journal of the American Veterinary Medical Association 205(11): 1588-1589

Delivery of a live calf after in vitro maturation and fertilization of oocytes obtained from ovaries removed from a moribund cow

An aged Holstein cow of high genetic merit and economic value was determined to have a squamous cell carcinoma of the tarsus. Treatment was unsuccessful, as was an attempt at superovulation and embryo collection; thus, the owner elected euthanasia. On day 17 of the estrous cycle, the ovaries were removed via laparotomy and the cow was immediately euthanatized. Oocytes were aspirated from ovarian follicles and subjected to in vitro maturation and fertilization procedures. Seven morulae that developed in culture were transferred nonsurgically to 3 recipient females, resulting in birth of a full-term healthy male calf. In vitro maturation and fertilization procedures can be used to obtain offspring from valuable, terminally ill cows for which conventional embryo collection techniques have proven unsuccessful.

(PDF 0-2 workdays service: $29.90)

Accession: 002590243

PMID: 7730130



Related references

Results obtained from pilot experiment for collection of oocytes from ovaries of slaughtered cattle for in vitro maturation and fertilization. Monatshefte fuer Veterinaermedizin 45(4): 138-141, 1990

In vitro maturation and fertilization of oocytes from unstimulated ovaries: Predicting the number of immature oocytes retrieved by early follicular phase ultrasonography. American Journal of Obstetrics & Gynecology 186(4): 684-689, April, 2002

In vitro maturation of follicular oocytes obtained pig ovaries at a slaughterhouse. Japanese Journal of Fertility & Sterility 34(4): 5-10, 1989

Potential uses of buffalo oocytes from ovaries with CL and without CL for in vitro maturation and fertilization. Buffalo Journal 21(3): 221-228, 2005

In vitro maturation and fertilization of oocytes from unstimulated normal ovaries, polycystic ovaries, and women with polycystic ovary syndrome. Fertility & Sterility 76(5): 936-942, November, 2001

Pup birth from mouse oocytes in preantral follicles derived from vitrified and warmed ovaries followed by in vitro growth, in vitro maturation, and in vitro fertilization. Fertility and Sterility 86(4 Suppl): 1182-1192, 2006

In vitro maturation and fertilization of bovine oocytes aspirated from the slaugthered ovaries. Ankara Universitesi Veteriner Fakultesi Dergisi 52(2): 93-98, 2005

A study of in vitro maturation and fertilization of oocytes from goat ovaries of different sources. Journal of Southwest Agricultural University 27(2): 197-200, 2005

Xenogenous fertilization of equine oocytes following recovery from slaughterhouse ovaries and in vitro maturation. Theriogenology 61(2-3): 381-391, 2003

In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries. Fertility and Sterility 81 Suppl 1: 824-830, 2004