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Demonstration of infectious salmon anaemia (ISA) viral antigens in cell cultures and tissue sections


, : Demonstration of infectious salmon anaemia (ISA) viral antigens in cell cultures and tissue sections. Veterinary Research 26(5-6): 499-504

Rabbit polyclonal antibodies and mouse monoclonal antibodies (MAb) directed against infectious salmon anaemia virus (ISAV) were produced using a virus prepared in a newly established cell culture developed from Atlantic salmon head kidney cells. The antibodies were used to establish an indirect fluorescent antibody test for the detection of viral antigens in cell cultures and tissue cryosections. Specific fluorescence was detected in ISAV-infected cell cultures using rabbit polyclonal and MAb.

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Accession: 002590354

PMID: 8581029

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