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Dengue virus premembrane and membrane proteins elicit a protective immune response

Virology 185(1): 505-508

Dengue virus premembrane and membrane proteins elicit a protective immune response

We have constructed recombinant vaccinia viruses that express the premembrane (pre-M), membrane (M), or the cleaved, residual portion of pre-M (non-M) protein of dengue 4 virus, or the pre-M, non-M, or envelope (E) proteins of dengue 2 virus, to evalute their ability to induce protective immunity in mice. Cells infected with these recombinants make proteins of expected size. Mice immunized with recombinants expressing dengue 4 pre-M or M were protected against subsequent dengue 4 encephalitis challenge, but non-M was not protective. A recombinant that expressed dengue 2 E was partially protective against homotypic challenge, but dengue 2 pre-M was not protective. However, a recombinant that expressed both pre-M and E as a polyprotein gave solid protection, while the simultaneous administration of the two recombinants expressing pre-M and E gave a significant level of protection. Pre-M and M function as antigens eliciting a protective immune response, and the combination of pre-M plus E is more protective than E alone.

Accession: 002590429

PMID: 1926792

DOI: 10.1016/0042-6822(91)90809-p

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