EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Derivatization reactions for the determination of aflatoxins by liquid chromatography with fluorescence detection


Journal of Chromatography B Biomedical Applications 659(1-2): 127-137
Derivatization reactions for the determination of aflatoxins by liquid chromatography with fluorescence detection
Various derivatization methods for the fluorometric detection of aflatoxins after separation by HPLC are reviewed. In normal-phase chromatography the sensitivity for aflatoxins B-1 and B-2, was improved by using special mobile phases or a flow cell packed with silica-gel particles. In the nowadays more popular reversed-phase methods, the fluorescence intensity of B-1 and G-1 can be increased by precolumn derivatization with trifluoroacetic acid or by postcolumn derivatization with iodine or bromine. Optimum conditions for the reactions are discussed. In terms of sensitivity, the three derivatization schemes give similar results. The methods are compared with respect to experimental convenience, selectivity, reproducibility and suitability for automation.

Accession: 002590621

PMID: 7820273

DOI: 10.1016/0378-4347(94)00152-9

Download PDF Full Text: Derivatization reactions for the determination of aflatoxins by liquid chromatography with fluorescence detection



Related references

Determination of aflatoxins by reversed-phase high-performance liquid chromatography with post-column in-line photochemical derivatization and fluorescence detection. Journal of Chromatography A 654(2): 247-254, 1993

Determination of aflatoxins in cereal flours by solid-phase microextraction coupled with liquid chromatography and post-column photochemical derivatization-fluorescence detection. Journal of Chromatography. A 1216(49): 8636-8641, 2010

Determination of aflatoxins B1, B2, G1, and G2 in olive oil, peanut oil, and sesame oil using immunoaffinity column cleanup, postcolumn derivatization, and liquid chromatography with fluorescence detection: first action 2013.05. Journal of Aoac International 96(5): 1017-1018, 2014

Determination of aflatoxins B1, B2, G1, and G2 in olive oil, peanut oil, and sesame oil using immunoaffinity column cleanup, postcolumn derivatization, and liquid chromatography/fluorescence detection: collaborative study. Journal of Aoac International 95(6): 1689-1700, 2013

Determination of low level aflatoxins in the pig urine iii. from the meat type hybrid pigs experimentally exposed to aflatoxins b 1 and g 1 by high pressure liquid chromatography with fluorescence detection. Journal of the Chinese Society of Veterinary Science 11(2): 175-182, 1985

Determination of nitrofuran metabolites in shrimp by high performance liquid chromatography with fluorescence detection and liquid chromatography-tandem mass spectrometry using a new derivatization reagent. Journal of Chromatography. A 1327: 90-96, 2014

An improved derivatization method for sensitive determination of fatty acids by high-performance liquid chromatography using 9-(2-hydroxylethyl)-carbazole as derivatization reagent with fluorescence detection. Chromatographia 54(5/6): 316-322, 2001

Simple derivatization method for sensitive determination of fatty acids with fluorescence detection by high-performance liquid chromatography using 9-(2-hydroxyethyl)-carbazole as derivatization reagent. Analytica Chimica Acta 436(1): 163-172, 2001

Ionic-liquid-based dispersive liquid-liquid microextraction combined with magnetic solid-phase extraction for the determination of aflatoxins B 1 , B 2 , G 1 , and G 2 in animal feeds by high-performance liquid chromatography with fluorescence detection. Journal of Separation Science 39(19): 3789-3797, 2016

Determination of four phenolic endocrine disruptors in environmental water samples by high performance liquid chromatography-fluorescence detection using dispersive liquid-liquid microextraction coupled with derivatization. Se Pu 32(6): 623-628, 2015