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Derivatization reactions for the determination of aflatoxins by liquid chromatography with fluorescence detection


, : Derivatization reactions for the determination of aflatoxins by liquid chromatography with fluorescence detection. Journal of Chromatography B Biomedical Applications 659(1-2): 127-137

Various derivatization methods for the fluorometric detection of aflatoxins after separation by HPLC are reviewed. In normal-phase chromatography the sensitivity for aflatoxins B-1 and B-2, was improved by using special mobile phases or a flow cell packed with silica-gel particles. In the nowadays more popular reversed-phase methods, the fluorescence intensity of B-1 and G-1 can be increased by precolumn derivatization with trifluoroacetic acid or by postcolumn derivatization with iodine or bromine. Optimum conditions for the reactions are discussed. In terms of sensitivity, the three derivatization schemes give similar results. The methods are compared with respect to experimental convenience, selectivity, reproducibility and suitability for automation.

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Accession: 002590621

PMID: 7820273

DOI: 10.1016/0378-4347(94)00152-9

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