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Detection of west Nile and Japanese encephalitis viral genome sequences in cerebrospinal fluid from acute encephalitis cases in Karachi, Pakistan

Igarashi, A.; Tanaka, M.; Morita, K.; Takasu, T.; Ahmed, A.; Ahmed, A.; Akram, D.S.; Waqar, M.A.

Microbiology and Immunology 38(10): 827-830

1994

ISSN/ISBN: 0385-5600
PMID: 7869964
DOI: 10.1111/j.1348-0421.1994.tb01866.x
Accession: 002591302
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Reverse transcriptase-polymerase chain reaction (RT-PCR) on 24 cerebrospinal fluid (CSF) specimens collected between February and August 1992 detected genome sequence of West Nile (WN) virus in 8 specimens and Japanese encephalitis (JE) virus in a single specimen. The results, combined with the data by IgM-ELISA on CSF indicated that a significant proportion of acute encephalitis cases in Karachi, Pakistan, were caused by WN virus infection, while JE virus caused a small fraction.

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