+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Detection of fetal twins in sheep using a radioimmunoassay for pregnancy-specific protein B



Detection of fetal twins in sheep using a radioimmunoassay for pregnancy-specific protein B



Journal of Animal Science 73(4): 960-966



Ovine pregnancy-specific protein B (oPSPB) was isolated from sheep placentas. Antiserum to oPSPB was developed in rabbits. A quantitative RIA was developed and used to assay the serum concentrations of oPSPB during and after pregnancy in ewes bearing single or twin fetuses. Suffolk and Panama ewes, kept with rams equipped with a marking harness, were checked daily for breeding marks as an indication of estrus and bled daily between 10 and 30 d after marking. Ovine PSPB became detectable at 19.7 +- .14 (mean +- SE) d after breeding and increased steadily to d 30. Panama oPSPB concentration increased at a greater rate than that of Suffolks (breed times day interaction, P lt .01). Ten ewes were bled twice weekly 3 wk before their expected date of lambing and weekly for 7 wk postpartum. Serum concentrations differed considerably between prepartum ewes, but concentrations remained stable within the period of 20 d prepartum. Following parturition, oPSPB concentrations dropped rapidly. In nine ewes, oPSPB was last detectable at 12.78 +- 2.26 (mean +- SE) d postpartum. In the 10th ewe, oPSPB was .65 ng/mL at the last sample on d 46 postpartum. To determine the effect of fetal number on oPSPB concentrations, ewes in which estrus was synchronized were bled at d 18, 25, 38, 60, 90, and 120 after breeding. Ewes were killed at d 60, 90, 120, and 148 and fetal number determined. There was a significant (P lt .01) difference in the log of oPSPB concentrations according to number of fetuses, day postbreeding, and the day times fetal number interaction. At 60, 90, and 120 d, the test was 82, 78, and 82% correct in detecting twins and single fetuses. With a combined 60 to 120 d of gestation range, the test was 78% correct at distinguishing twin from single pregnancies. The RIA provided values that were predictive. Ovine PSPB was detectable in sera very early in gestation, was not detectable within a few weeks after parturition, and was unusually higher in ewes bearing twins.

(PDF emailed within 1 workday: $29.90)

Accession: 002591440

Download citation: RISBibTeXText

PMID: 7628973


Related references

Detection of pregnancy in sheep by radioimmunoassay of sera for pregnancy-specific protein B. Theriogenology 29(4): 905-912, 1988

Pregnancy detection in farm animals by radioimmunoassay of a pregnancy specific protein in serum. Hau, J (Ed ) Pregnancy Proteins in Animals; International Meeting, Copenhagen, Denmark, April 22-24, 1985 Xi+536p Walter De Gruyter And Co : Berlin, West Germany; New York, New York, Usa Illus 511-517, 1986

Detection of pregnancy by radioimmunoassay of a novel pregnancy specific protein in serum of cows and a profile of serum concentrations during gestation. Biology of Reproduction 35(4): 936-942, 1986

Early pregnancy detection in mountain sheep using a pregnancy-specific protein B assay. Wildlife Society Bulletin 29(4): 1182-1185, 2001

Detection of pregnancy in sheep using an ELISA for pregnancy-specific protein B. Acta Veterinaria Hungarica 66(2): 329-336, 2018

Detection of pregnancy-specific beta 1-glycoproteins (SP1) and pregnancy-associated plasma protein A (PAPP-A) in maternal serum. Diagnostic significance in intrauterine fetal retardation. Zentralblatt für Gynakologie 106(8): 517-523, 1984

A serum pregnancy test with a specific radioimmunoassay for moose and elk pregnancy-specific protein B. Journal of Wildlife Management 64(2): 492-499, 2000

Validation of a specific radioimmunoassay to measure plasma cortisol in the fetal sheep. Steroids 33(1): 45-53, 1979

Diagnosis of pregnancy by radioimmunoassay of a pregnancy-specific protein in the plasma of dairy cows. Theriogenology 30(2): 257-267, 1988

Transabdominal ultrasound for detection of pregnancy, fetal and placental landmarks, and fetal age before Day 45 of gestation in the sheep. Theriogenology 85(5): 939-45.E1, 2015

Detection of pregnancy by radioimmunoassay of a pregnancy serum protein (PSP60) in cattle. Reproduction, Nutrition, Development 34(1): 65-72, 1994

Pregnancy determination by use of pregnancy-specific protein B radioimmunoassay in llamas. Journal of the American Veterinary Medical Association 207(2): 217-219, 1995

Detection of pregnancy-specific beta 1-glycoprotein (SP1), of proteins of the "pregnancy zone" (PZ), of pregnancy-associated plasma protein A (PAPP-A) with 24-hour radial immunodiffusion. Detection of pregnancy-specific beta 1-glycoprotein (SP1) with turbidimetry. Current significance of the proteins. Zentralblatt für Gynakologie 105(8): 516-526, 1983

Clinical analysis of pregnancy outcomes and fetal loss after fetal reduction of triplets to twins or singleton pregnancy. Zhonghua Fu Chan Ke Za Zhi 50(4): 268-273, 2015

Isolation and radioimmunoassay of a bovine pregnancy specific protein. Theriogenology 29(1): 219, 1988