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Effect of complex nitrogen sources on pullulan production relative to carbon source

, : Effect of complex nitrogen sources on pullulan production relative to carbon source. Microbios 80(323): 83-90

The effect of complex nitrogen sources, including tryptone, peptone, soytone, casamino acids and corn steep liquor, on pullulan production by Aureobasidium pullulans ATCC 42023 was studied in a medium where sucrose or corn syrup served as the carbon source. Growth on soytone resulted in the highest level of pullulan production by ATCC 42023 independent of the carbon source utilized. In general, all the complex nitrogen sources examined increased pullulan production relative to ammonium sulphate for either sucrose- or corn syrup-grown cells. Cell weights of ATCC 42023 grown on either carbon source were lower when the culture medium contained a complex nitrogen source instead of ammonium sulphate. The pullulan content of the polysaccharide elaborated by sucrose-grown cells on day 5 was found to be highest on soytone, peptone or corn steep liquor. The pullulan content of the polysaccharide produced by corn syrup-grown cells on day 5 was shown to be highest on peptone. The lowest pullulan content of the polysaccharide synthesized by the fungus, using either carbon source, was found to occur after growth on casamino acids.

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