+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Effect of cycloheximide on eclosion hormone sensitivity and the developmental appearance of the eclosion hormone and cGMP regulated phosphoproteins in the CNS of the tobacco hornworm, Manduca sexta



Effect of cycloheximide on eclosion hormone sensitivity and the developmental appearance of the eclosion hormone and cGMP regulated phosphoproteins in the CNS of the tobacco hornworm, Manduca sexta



Journal of Receptor and Signal Transduction Research 15(5): 773-786



The neuropeptide, eclosion hormone (EH), triggers ecdysis behavior at the end of each molt in Manduca sexta. Previous studies have shown that the action of EH is mediated by an increase in cGMP and is associated with the phosphorylation of two proteins, named the EGPs. The ability of insects to respond to EH is developmentally regulated with sensitivity being first seen at about 8 hr prior to the normal time of ecdysis. The EGPs are also first detectable in the CNS at 8 hr prior to ecdysis, suggesting that it is their synthesis which determines EH sensitivity. The protein synthesis inhibitor, cycloheximide was used to study the development of the events leading to pupal ecdysis in Manduca. The results of these experiments suggest that protein synthesis is necessary about 10 hr before ecdysis for both the development of EH sensitivity and for the appearance of the EGPs.

(PDF emailed within 0-6 h: $19.90)

Accession: 002600824

Download citation: RISBibTeXText

PMID: 8747886

DOI: 10.3109/10799899509079906



Related references

The egps eclosion hormone and cyclic gmp regulated phosphoproteins in the cns of manduca sexta effects of cycloheximide and actinomycin d. Society for Neuroscience Abstracts 14(1): 29, 1988

The EGPs: the eclosion hormone and cyclic GMP-regulated phosphoproteins. II. Regulation of appearance by the steroid hormone 20-hydroxyecdysone in Manduca sexta. Journal of Neuroscience 8(4): 1338-1345, 1988

The EGPs: the eclosion hormone and cyclic GMP-regulated phosphoproteins. I. Appearance and partial characterization in the CNS of Manduca sexta. Journal of Neuroscience 8(4): 1326-1337, 1988

The role of eclosion hormone in the development of the tobacco hornworm manduca sexta. American Zoologist 20(4): 862, 1980

Isolation of the eclosion hormone gene from the tobacco hornworm manduca sexta. Journal of Cell Biology 107(6 PART 3): 508A, 1988

Isolation and primary structure of the eclosion hormone of the tobacco hornworm, Manduca sexta. Biochemical and Biophysical Research Communications 146(2): 746-750, 1987

Isolation and expression of the eclosion hormone gene from the tobacco hornworm, Manduca sexta. Proceedings of the National Academy of Sciences of the United States of America 86(20): 8123-8127, 1989

Microanalysis of the amino acid sequence of the eclosion hormone from the tobacco hornworm Manduca sexta. Febs Letters 219(2): 415-418, 1987

Ecdysteroids regulate the release and action of eclosion hormone in the tobacco hornworm, Manduca sexta (L.). Journal of Insect Physiology, 2912: 895-900, 1983

The molecular basis for steroid regulation of eclosion hormone action in the tobacco hornworm, Manduca sexta. Studies in Neuroscience, 187-197 No. 10, 1991

Physiology of pupal ecdysis in the tobacco hornworm, Manduca sexta I. Evidence for control by eclosion hormone. Journal of Experimental Biology 88: 327-337, 1980

Identification of the cellular target for eclosion hormone in the abdominal transverse nerves of the tobacco hornworm, Manduca sexta. Journal of Comparative Neurology 424(2): 339-355, August 21, 2000

Regulation of behavioral sensitivity to the neuropeptide eclosion hormone is different in the adult and pupal stages of the tabacco hornworm manduca sexta. Society For Neuroscience Abstracts: 1255, 1987

Physiology of pupal ecdysis in the tobacco hornworm, Manduca sexta II Chemistry, distribution, and release of eclosion hormone at pupal ecdysis. Journal of Experimental Biology 88: 339-349, 1980