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Effect of salinity on the cell structure in different genotypes of tomato produced by tissue culture



Effect of salinity on the cell structure in different genotypes of tomato produced by tissue culture



Sarhad Journal of Agriculture 10(3): 321-325



This investigation was conducted to determine the influence of salinity on the cell structure during the growth. Three cultivars, namely Beijing Early, Tropics, Floredal, and one wild specie Lycopercicon pimpinellifolium were produced by Tissue culture (callus culture) at different concentrations of NaCl (0.0%, 0.2%, 0.4%, 0.5%, 0.6%, 0.8%, 1.0%). Plants were transformed in the Green House at the same levels of NaCl in the Hoagland nutrient solution. With the increase of salinity a significant increase in cell conductivity was found in all the genotypes. Electron microscopic studies revealed that there was no change in grana in Beijing Early at control and in L. pimpinellifolium at 0.8% level NaCl. In Beijing Early grana was abnormal at 0.4% and missing at 1.0% level of NaCl. Osmopholic globular bodies and starch grains in L. pimpinellifolium, at 0.8% level of NaCl were many and large in size, while in Beijing Early they were missing at 1.0% level of NaCl. In Beijing Early shape of chloroplast was changed at different concentrations of NaCl while in L. pimpinellifolium there was no change. Salinity changed the thylakoids order in all the genotypes.

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Accession: 002604224

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