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Electron spin resonance spin trapping studies of the effects of dietary zinc deficiency on free radical production in vitro and in vivo under acute oxidative stress



Electron spin resonance spin trapping studies of the effects of dietary zinc deficiency on free radical production in vitro and in vivo under acute oxidative stress



Journal of Nutritional Biochemistry 5(10): 490-494



The objective of this study was to investigate the effect of dietary zinc deficiency on free radical production in the rat liver homogenate and whole liver under acute oxidative stress in vitro and in vivo. Three-week-old male Wistar rats were given free access to either a zinc-adequate diet or a zinc-deficient diet for 3 weeks. A second control group, the pair-fed control, was also included. The oxidative stress was imposed in vitro by incubating whole-liver homogenate prepared from rats with CCl-4, ethanol, or FeNTA. The same oxidants were used to impose oxidative stress in vivo. Incubation of liver homogenate with CCl-4 or ethanol resulted in the detection of their free radical metabolites, a CCl-3 cntdot radical (alpha-N = 13.9 G, alpha-beta-H = 1.9mG) and a 1-hydroxyethyl radicalm(alpha-N =m15.1mG, alpha-beta-H = 4.6 G), respectively. A free radical (alpha-N = 14.9 G, alpha-beta-H = 4.8 G) was detected in the whole-liver homogenate incubated with FeNTA. A CCl-3 cntdot radical was detected in the livers of CCl-4-challenged rats in vivo. However, no free radicals were detected in the livers of ethanol- or FeNTA-challenged rats regardless of dietary treatment. The production of free radicals both in vitro and in vivo was not affected by dietary zinc deficiency as indicated by electron spin resonance signals of alpha-phenyl-N-tert-butyl nitrone-spine adduct. It seems that dietary zinc-deficiency rats are capable of coping with an increased free radical production in liver, both in vitro and in vivo, under acute oxidative stresses.

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Accession: 002610929

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DOI: 10.1016/0955-2863(94)90028-0


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