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Electroporation of wheat anther culture-derived embryoids

Electroporation of wheat anther culture-derived embryoids

Cereal Research Communications 23(3): 207-213

Electroporation, a method of DNA delivery successfully used to transform other cereal crops, shows potential to be a useful method for wheat transformation. The goal of this research was to identify the electroporation parameters that would result the highest number of regenerated plants and evaluate selection criteria for transformation. Anther culture-derived embryoids were electroporated at 500 and 960 mu-F with and without an enzyme pretreatment. Electroporation at 500 mu-F produced more regenerable embryoids than electroporation at 960 mu-F. The plasmid used for electroporation and transformation selection contained the uidA (GUS) and bar genes encoding for beta-glucuronidase and phosphinothricin acetyl transferase which imparts resistance to the herbicide Basta. Of the 66 plants regenerated thus far, one has expressed GUS activity. None have expressed Basta resistance.

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