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Endogenous gibberellins from callus cultures of maize



Endogenous gibberellins from callus cultures of maize



Phytochemistry (Oxford) 40(1): 11-15



This paper reports the identification of gibberellin A-1(GA-1), GA-17, GA-19, GA-20, GA-29, and GA-53 from extracts of normal (wild-type) maize callus using full-scan GC-Mass Spectrometry and Kovats retention indices (KRIs). In addition, evidence is presented for the presence of GA-8 based on its molecular ion at the appropriate KRI. In a preliminary experiment, the presence of GA-3, GA-5, and GA-44 was suggested, based on GC-selected ion monitoring (SIM); however, the presence of these GAs was not confirmed by full-scan GC-Mass Spectrometry. The information represents the first identification of GAs from maize callus. All of the identified GAs are members of the early-13-hydroxylation pathway, a biosynthetic pathway that leads to bioactive GA-1, GA-1, GA-8, GA-19, GA-20, and GA-29 were quantified from GC-SIM isotopic dilution data. GA-17 and GA-53 were quantified from GC-SIM data by comparing the ion intensity from each gibberellin to a known amount of a GA-19 standard. The levels of the identified GAs ranged from 6 pg g-1 fr. wt to 210 pg g-1 fr. wt.

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Accession: 002611545

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DOI: 10.1016/0031-9422(95)00000-w



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