Establishment of embryogenic cell suspension cultures from mature embryos of barley (Hordeum vulgare L.) and plantlet regeneration
Xiang YouBin; Liu Jing; Ruan YiLi; Yuan AiMin
Acta Agriculturae Zhejiangensis 5(4): 213-219
Embryogenic calli were induced from mature embryos of barley Zaosu 3, Supi 1, Yong 257, Interbell and 83055, and cell suspension cultures were initiated from these calli in various media. Eight fast-growing homogeneous cell suspensions were established; 5 of these regenerated green plantlets, 2 produced albino plantlets and 1 did not show morphogenic ability. AA and MS media were better than L3 and B5 media for callus growth.