+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Improved detection of Candida albicans by PCR in blood of neutropenic mice with systemic candidiasis



Improved detection of Candida albicans by PCR in blood of neutropenic mice with systemic candidiasis



Journal of Clinical Microbiology 33(3): 625-628



A PCR using primers aimed at the multicopy gene coding for the small subunit rRNA and resulting in the synthesis of a 180-bp fragment was evaluated for its use in diagnosing invasive candidiasis in comparison with blood culture. With the use of a C. albicans-specific probe, +/- 10 to 15 C. albicans cells are detected in 100 microliters of whole blood by Southern analysis. A DNase pretreatment was critical in the purification process of yeast DNA from whole blood. Omission of the DNase pretreatment decreased assay sensitivity 10-fold. PCR analysis of blood specimens collected from mice with invasive candidiasis is more sensitive than blood culture (100 versus 67%, respectively) at 72 h after intravenous (i.v.) inoculation with C. albicans. Furthermore, the intensity of the hybridization signals increased with the progression of infection. In contrast, multiple blood samples from gastrointestinally colonized mice were all negative by PCR, indicating that the PCR assay is also specific and may, therefore, make a positive contribution to the detection and follow-up of invasive candidiasis.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 002635709

Download citation: RISBibTeXText

PMID: 7751367


Related references

Detection of antibodies to Candida albicans germ tube by immunofluorescence in immunosuppressed mice with experimental systemic candidiasis. 2007

Systemic candidiasis in mice immunized with Candida albicans ribosomes. Mycopathologia 91(1): 17-22, 1985

Systemic candidiasis from Candida albicans colonizing the gastrointestinal tract of mice. Experientia 35(10): 1331-1332, 1979

Isolation and characterization of 65 kd antigen from Candida albicans and its detection in systemic candidiasis patients. Abstracts of the General Meeting of the American Society for Microbiology 94: 626, 1994

Detection of antibodies to Candida albicans germ tube in the diagnosis of systemic candidiasis. European Journal of Clinical Microbiology 6(2): 142-146, 1987

Standardized immunoenzyme analysis for detection of IgG antibodies against Candida albicans in systemic candidiasis. Enfermedades Infecciosas Y Microbiologia Clinica 10(4): 195-199, 1992

Comparison of the efficacies of amphotericin B, fluconazole, and itraconazole against a systemic Candida albicans infection in normal and neutropenic mice. Antimicrobial Agents and ChemoTherapy 33(2): 147-151, 1989

Detection of an antibody response in immunocompetent patients with systemic candidiasis or Candida albicans colonisation. European Journal of Clinical Microbiology & Infectious Diseases 9(5): 352-355, 1990

Detection of antibodies to Candida albicans germ tubes in heroin addicts with systemic candidiasis. Clinical Microbiology and Infection 7(4): 224-226, 2001

Improved detection of deeply invasive candidiasis with DNA aptamers specific binding to (1→3)-β-D-glucans from Candida albicans. European Journal of Clinical Microbiology and Infectious Diseases 35(4): 587-595, 2016

Detection of antibodies to Candida albicans germ tube in heroin addict patients with systemic candidiasis. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 37: 106, 1997

In vivo efficacies of pradimicin derivatives, BMS-181184 and BMY-28864, and benanomicin A against systemic Candida albicans and Aspergillus fumigatus infections in neutropenic mice. Japanese Journal of Medical Mycology 34(4): 471-476, 1993

Detection of antibodies to Candida albicans germ tube as a possible aid in diagnosing systemic candidiasis in bone marrow transplant patients. European Journal of Clinical Microbiology & Infectious Diseases 12(5): 347-349, 1993