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Lessened malate inhibition of guard-cell phosphoenolpyruvate carboxylase velocity during stomatal opening



Lessened malate inhibition of guard-cell phosphoenolpyruvate carboxylase velocity during stomatal opening



FEBS Letters 352(1): 45-48



Leaflets of Vicia faba with closed stomata or with opening stomata were freeze-dried. Excised guard-cell pairs were assayed individually under suboptimal conditions (pH 7.1 and subsaturating substrate) for phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) using quantitative histochemical procedures. L-Malate, 400, mu-M, significantly inhibited guard-cell PEPC activity of closed stomata but not that of opening stomata. We postulate that the lessened sensitivity of guard-cell PEPC activity to malate inhibition is an important regulatory feature of stomatal opening, which is associated with malate accumulation.

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Accession: 002647966

Download citation: RISBibTeXText

PMID: 7925940

DOI: 10.1016/0014-5793(94)00916-3



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