Liver ascorbate and glutathione disulfide levels in guinea pigs are affected by dietary ascorbate intake and the type of diet

Netke, S.; Niedzwiecki, A.

International Journal for Vitamin and Nutrition Research. Internationale Zeitschrift für Vitamin- und Ernahrungsforschung. Journal International de Vitaminologie et de Nutrition 65(3): 187-192


ISSN/ISBN: 0300-9831
PMID: 8829998
Accession: 002649092

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We studied effects on the levels and oxidant status of AA and GSH in the guinea pig liver. Group I received various doses of AA supplementation in a standard diet, Group II in a "western" high fat, high sugar, high cholesterol diet. Both diets were supplemented with 1 (Ia and IIa); 25, (Ib and IIb); and 1000, (Ic and IIc) mg AA per animal per day. The Group II animals were sacrificed during the 9th and 10th weeks, those in Group I during the 19th and 20th weeks. Liver AA levels were 30 and 26 micrograms/g in subgroups Ia and IIa, 367 and 282 micrograms/g in subgroups Ib and IIb, and 604 and 481 micrograms/g in subgroups Ic and IIc. In subgroups Ia and IIa most of the AA was detected as DHAA. DHAA and AA levels were related in a biphasic manner. GSSG content tended to increase with higher intakes of AA regardless of diet and ranged between 0.094 to 0.194 mumol/g. GSH levels were not affected by diet type or AA level. GSSG and DHAA levels were affected only by dietary AA. Changes in serum TBARS suggested a preventive effect of higher dietary ascorbate on lipid peroxidation, but only with a western type diet.