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Long-term shoot regeneration from pampas grass (Cortaderia selloana Schult.) through manipulation of growth regulators in vitro


Long-term shoot regeneration from pampas grass (Cortaderia selloana Schult.) through manipulation of growth regulators in vitro



Plant Cell Reports 14(11): 689-693



ISSN/ISBN: 0721-7714

PMID: 24186623

DOI: 10.1007/bf00232648

A regeneration system for 'Pumila' pampas grass (Cortaderia selloana Schult.) that yields plants over many months and allows control of morphogenesis was developed. Immature inflorescence explants cultured for three 4-week passages on MS basal medium supplemented with 4.5 μM 2,4-D and 8.9 μM BA yielded a dark green callus that organized into shoot primordia. Rate of shoot development was increased after transfer of shoot primordia to medium supplemented with 9.8 μM IBA. Subculture every 4-6 weeks onto medium containing IBA yielded a continuous production of shoots. Control of morphogenesis was achieved by transferring cultures to medium containing 4.5 μM 2,4-D and 8.9 μM BA for shoot bud proliferation and to medium containing IBA for shoot production.

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Accession: 002649680

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Related references

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