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Molecular cloning and nucleotide sequencing of bovine viral diarrhoea virus gp53 antigenic region


Molecular cloning and nucleotide sequencing of bovine viral diarrhoea virus gp53 antigenic region



Korean Journal of Veterinary Research 35(2): 287-295



ISSN/ISBN: 2466-1384

The RNA genome of gp53 antigenic region in cytopathic Singer strain of bovine viral diarrhoea virus was cloned and the nucleotide sequence was examined. The cloned cDNA was 939 nucleotides in length, having a base composition of 31.0% A, 19.6% C, 25.5% G and 24.0 T. The sequence corresponded to approximately 77.8% (817 bases) of the predicted gp53 region and 122 bases after 3' end of the gp53 region in the Singer strain when compared with the NADL strain of known sequence. A single open reading frame was found in the sequence of the 2nd frame and was deduced to encode 312 amino acids.

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Accession: 002656791

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