Pathological studies on a densonucleosis virus (China strain) of the silkworm, Bombyx mori

Iwashita, Y.; Tsurubuchi, Y.; Ansai, K.; Kanke, E.; Jian, H.C.; Nanba, A.; Seki, H.

Bulletin of the College of Agriculture, Utsunomiya University 15(3): 21-69

1994


Accession: 002668525

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 1 workday
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
The results are presented of a laboratory investigation on the China strain (CDNV) of a densonucleosis virus (Bm DNV) of Bombyx mori. Special attention was paid to the replication pattern, nucleic acids, structural proteins, differences in specific antigens to viruses and methods for diagnosing infection. CDNV develops in the nuclei of columnar cells of the mid- and hind-portions of the midgut of the host. Protein synthesis is activated in the cytoplasm of infected cells. At late stages of infection, infected columnar cells swell to 2-3 times as large as the normal nuclei and are completely filled with virions, which are released into the midgut cavity. Defensive reactions are reported. In mixed infections with DNV and nuclear polyhedrosis or cytoplasmic polyhedrosis viruses, interference between CDNV and nuclear polyhedrosis viruses was observed. CDNV was considered to be the same strain as BmDNV-2. Histopathological diagnosis of a part of the midgut of infected larvae with pyronine-methyl green by the smear or vital staining method was effective in determining DNV infection at an early stage. In addition, a Dot-IGSS method was able to detect to 1 ng of virus and it was possible to diagnose the infection with 1 micro l of the homogenised tissue for 10 h after the DNV infection.