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Polymerase chain reaction (PCR) for the diagnosis of naturally occurring feline coronavirus infections

Polymerase chain reaction (PCR) for the diagnosis of naturally occurring feline coronavirus infections

Feline Practice 23(3): 56-60

A nested reverse transcriptase PCR (RT-nPCR) assay was developed for the detection of feline coronavirus (FCoV) RNA in faeces, tissues, and body fluids of infected cats. The assay was targeted to the 3' nontranslated region of the FCoV genome and detected most FCoV field strains. The assay was highly sensitive and detected FCoV RNA in the plasma of experimentally infected cats from 2 days after inoculation onward.

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Accession: 002673851

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