+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Porcine respiratory coronavirus-mediated interference against influenza virus replication in the respiratory tract of feeder pigs



Porcine respiratory coronavirus-mediated interference against influenza virus replication in the respiratory tract of feeder pigs



American Journal of Veterinary Research 55(9): 1275-1281



Effect of prior porcine respiratory coronavirus (PRCV) infection on replication of H1N1-influenza virus in the respiratory tract of swine was studied. In an initial experiment, 3 groups of 5 feeder pigs were studied. Pigs of 2 groups were inoculated sequentially with PRCV, followed by H1N1-influenza virus at 2- and 3-day intervals. Pigs of the other group were inoculated with H1N1-influenza virus only. Pigs were monitored clinically and examined for nasal excretion of influenza virus. In the singly influenza virus-inoculated group, 83% of nasal swab specimens were influenza virus-positive over a period of 6 days after inoculation. In the dually virus-inoculated groups, only 27% (2-day interval) and 53% (3-day interval) of nasal swab specimens were virus-positive over the same postinoculation period. However, clinical signs of infection in these dually inoculated pigs were more severe than those in the singly influenza virus-inoculated pigs. There were no significant differences in antibody responses against influenza virus among the 3 groups of pigs. In a second experiment, 2 groups of pigs were studied. One group of pigs was inoculated sequentially with PRCV, followed by H1N1-influenza virus 2 days later; the other group was inoculated with H1N1-influenza virus only. Pigs of both groups were serially euthanatized on postinoculation days (PID) 1, 2, 3, and 4 (after influenza virus). At necropsy, influenza virus titer and immunofluorescence in lung tissue were determined and gross lung lesions were recorded. Influenza virus titer in the dually inoculated pigs (PID 1 and 2) was at least 100-fold reduced, compared with that in the corresponding singly inoculated pigs, and fluorescence was either not detected (PID 1) or was scant (PID 2). Differences in influenza virus replication between pigs of dually and singly inoculated groups became gradually less pronounced at PID) 3 and 4. Lung lesions in the dually virus-inoculated pigs were distinctly more severe than those in the corresponding singly virus-inoculated pigs, and became progressively more pronounced as time after influenza virus inoculation progressed. These results indicate that PRCV infection may induce factors in the lungs that markedly interfere with replication and virus production during a subsequent influenza virus infection. On the other hand, clinical signs of infection and lung lesions were enhanced in the dually virus-inoculated pigs. It is believed that early nonspecific defense mechanisms in the lungs may have a role in the host antiviral response, as well as in development of lesions.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 002674396

Download citation: RISBibTeXText

PMID: 7802396


Related references

Dual infections of feeder pigs with porcine reproductive and respiratory syndrome virus following by porcine respiratory coronavirus or swine influenza virus: A clinical and virological study. Veterinary Microbiology 48(3-4): 325-335, 1996

Nitric oxide is elicited and inhibits viral replication in pigs infected with porcine respiratory coronavirus but not porcine reproductive and respiratory syndrome virus. Veterinary Immunology and Immunopathology 136(3-4): 335-339, 2010

Porcine reproductive and respiratory syndrome virus modifies innate immunity and alters disease outcome in pigs subsequently infected with porcine respiratory coronavirus: implications for respiratory viral co-infections. Journal of General Virology 90(Pt 11): 2713-2723, 2009

Pathogenicity of concurrent infection of pigs with porcine respiratory coronavirus and swine influenza virus. Research in Veterinary Science 53(3): 309-314, 1992

Porcine reproductive and respiratory syndrome virus-induced immunosuppression exacerbates the inflammatory response to porcine respiratory coronavirus in pigs. Viral Immunology 23(5): 457-466, 2010

Inhibition of porcine reproductive and respiratory syndrome virus replication by adenovirus-mediated RNA interference both in porcine alveolar macrophages and swine. Antiviral Research 82(3): 157-165, 2009

Inhibition of highly pathogenic porcine reproductive and respiratory syndrome virus replication by recombinant pseudorabies virus-mediated RNA interference in piglets. Veterinary Microbiology 181(3-4): 212-220, 2015

Altered pathogenesis of porcine respiratory coronavirus in pigs due to immunosuppressive effects of dexamethasone: implications for corticosteroid use in treatment of severe acute respiratory syndrome coronavirus. Journal of Virology 81(24): 13681-13693, 2007

H7N9 Influenza A Virus Exhibits Importin-α7-Mediated Replication in the Mammalian Respiratory Tract. American Journal of Pathology 187(4): 831-840, 2017

Sites of replication of a porcine respiratory coronavirus in 5-week-old pigs with or without maternal antibodies. Advances in Experimental Medicine and Biology 276: 429-433, 1990

Respiratory symptoms in meat pigs: is there a role for porcine respiratory corona virus (PRCV) and porcine reproductive and respiratory syndrome virus (PRRSV) in the etiology?. Tijdschrift Voor Diergeneeskunde 122(16): 434-436, 1997

Isolation of porcine respiratory coronavirus from pigs affected with porcine reproductive and respiratory syndrome. Journal of Veterinary Medical Science 58(4): 385-388, 1996

Respiratory tract protection upon challenge of pigs vaccinated with attenuated porcine reproductive and respiratory syndrome virus vaccines. Veterinary Microbiology 95(3): 187-197, 2003

Inhibition of replication and infection of severe acute respiratory syndrome-associated coronavirus with plasmid-mediated interference RNA. Antiviral Therapy 10(4): 527-533, 2005

Expression of inflammatory cytokines and Toll-like receptors in the brain and respiratory tract of pigs infected with porcine reproductive and respiratory syndrome virus. Veterinary Immunology and Immunopathology 135(3-4): 314-319, 2010