EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Production of transgenic mice by microinjection of DNA into vitrified pronucleate stage eggs


Production of transgenic mice by microinjection of DNA into vitrified pronucleate stage eggs



Transgenic Research 4(3): 208-213



ISSN/ISBN: 0962-8819

PMID: 7795663

DOI: 10.1007/bf01968786

Vitrification is a technique for cryopreserving cells without crystallization due to elevation of the viscosity during the cooling process. We have developed a rapid and convenient mean of cryopreserving mouse preimplantation embryos by vitrification using a solution (hereafter named DPS) consisting of 2.75 M dimethylsulfoxide, 2.75 M propylene glycol and 1.0 M sucrose. In vitro fertilized pronucleate stage eggs were used because a large number of stage-matched eggs can be obtained at once. Only successfully fertilized eggs were collected and vitrified in DPS. After warming, two DNA constructs were injected into a total of 257 cryopreserved eggs, of which 175 (68%) survived the injection and were transferred into six recipients. All recipients became pregnant and gave birth to a total of 20 pups. When these DNA constructs were concomitantly injected into fresh eggs, 18% of eggs that were transferred developed into live pups, which was the same as the 18% figure for the cryopreserved eggs. With respect to transgenesis, 40% of the pups (8/20) developed from vitrified eggs were transgenic. In terms of the injected eggs that had been transferred, 4.5% of the 213 fresh eggs and 3.1% of the 112 vitrified eggs developed into transgenic mice. These results indicate that the efficiency of production of transgenic mice from vitrified eggs is comparable to that from fresh eggs.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002678615

Download citation: RISBibTeXText

Related references

Production of transgenic mice from vitrified pronuclear-stage embryos. Molecular Reproduction and Development 61(2): 173-179, 2002

The production of transgenic mice by the microinjection of cloned dna into fertilized one cell eggs. Glover, D M (Ed ) Practical Approach Series: Dna Cloning, Vol Iii A Practical Approach Xviii+254p Irl Press Ltd : Oxford, England, Uk; Washington, D C , Usa Illus 213-248, 1987

Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice. Biology Open 6(5): 706-713, 2017

Production of transgenic rodents by the microinjection of cloned DNA into fertilized one-cell eggs. Molecular Biotechnology 17(2): 151-182, 2001

Production of transgenic rodents by the microinjection of cloned DNA into fertilized one-celled eggs. Methods in Molecular Biology 461: 71-109, 2008

Production of transgenic rodents by the microinjection of cloned DNA into fertilized one-celled eggs. Methods in Molecular Biology 97: 61-100, 1999

Production of the transgenic ice goby , Leucopsarion petersii, by microinjection of the GFP-expression vector into fertilized eggs. Zoological Science 15(Suppl. ): 22, 1998

Production of the transgenic ice goby , Leucopsarion petersii, by microinjection of the GFP-expression vector into unfertilized or fertilized eggs. Zoological Science 16(Suppl ): 77, 1999

Production of transgenic tilapia (Oreochromis niloticus) by one-cell-stage microinjection. Aquaculture, 1053-4: 219-232, 1992

The production of transgenic mice by embryo microinjection. Genetic Analysis Techniques and Applications 8(3): 83-94, 1991

Production of transgenic mice by pronuclear microinjection. Celis, J E Cell biology, Vol 3 Second edition 473-477, 1998

Production of Transgenic Mice by Pronuclear Microinjection. Methods in Molecular Biology 1874: 17-41, 2019

Production of transgenic mice rabbits and pigs by microinjection. Theriogenology 25(1): 143, 1986

Production of transgenic mice following dna microinjection and embryo freezing. Theriogenology 27(3): 507-516, 1987

Production of functional transgenic mice by DNA pronuclear microinjection. Acta Biochimica Polonica 51(1): 9-31, 2004