+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Pseudorecombination and complementation between potato yellow mosaic geminivirus and tomato golden mosaic geminivirus

Pseudorecombination and complementation between potato yellow mosaic geminivirus and tomato golden mosaic geminivirus

Journal of General Virology 76: 2809-2815

Pseudorecombinants made by exchanging the cloned, infectious genome components (DNAs A and B) of potato yellow mosaic geminivirus (PYMV) and the common strain (cs) of tomato golden mosaic geminivirus (csTGMV) are not infectious in their common host Nicotiana benthamiana. In an N. benthamiana leaf disc assay neither PYMV DNA A nor TGMV DNA A transreplicated each other's DNA B component. The ability of PYMV and TGMV to mediate the systemic movement of each other's DNA A was investigated following co-inoculation of N. benthamiana with both genome components of one virus (the helper virus) and DNA A of the other virus (the dependent virus). Movement of the dependent virus DNA A in both cases illustrates interchangeability between the DNA B-encoded movement proteins of New World geminiviruses which infect solanaceous hosts. We have studied this genetic interchangeability further in separate co-agroinoculation experiments with N. benthamiana plants using TGMV DNA A to complement mutations in PYMV open reading frame (ORF) AC2, which encodes a protein that trans-activates the expression of virion sense promoters, and in PYMV ORF AC3, which specifies a protein that enhances viral DNA replication. TGMV DNA A complemented a PYMV AC2 mutant and restored its infectivity and it also complemented a PYMV AC3 mutant and restored the reduced DNA phenotype.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 002680369

Download citation: RISBibTeXText

PMID: 7595388

DOI: 10.1099/0022-1317-76-11-2809

Related references

Molecular cloning of cowpea golden mosaic geminivirus and its relationship with mungbean yellow mosaic geminivirus. Tropical Agricultural Research 13: 341-352, 2001

Natural infection of potato in Venezuela by tomato yellow mosaic, a geminivirus transmitted by whiteflies. Agronomia Tropical 35(1-3): 125-138, 1985

Occurrence of a strain of potato yellow mosaic geminivirus infecting tomato in the Eastern Caribbean. Plant Disease 82(1): 126, 1998

Macroptilium lathyroides infected by a geminivirus distinct from bean golden mosaic geminivirus in the Dominican Republic. Annual report of the Bean Improvement Cooperative5(35): 152-153, 1992

Inhibition of African cassava mosaic virus systemic infection by a movement protein from the related geminivirus tomato golden mosaic virus. Virology 187(2): 555-564, 1992

Characterisation of tomato golden mosaic virus as a geminivirus. Fems Microbiology Letters 11(4): 263-267, 1981

Serological studies on tomato golden mosaic virus, a geminivirus. Journal of general virology 64(11): 2493-2498, 1983

Properties of tomato yellow mosaic geminivirus in Venezuela. Phytopathology 86(11 SUPPL ): S114, 1996

Mutational analysis of potato yellow mosaic geminivirus. Journal of General Virology 76: 1773-1780, 1995

Properties of replication proteins encoded by the geminivirus Tomato Golden Mosaic Virus. Experientia (Basel) 49(ABSTR ): A4, 1993

The infectivity of dimeric potato yellow mosaic geminivirus clones in different hosts. Journal of General Virology 75: 2857-2861, 1994

Characterization of potato yellow mosaic virus as a geminivirus with a bipartite genome. Intervirology 29(3): 162-169, 1988

Resistance to tomato yellow mosaic geminivirus and presence of viral DNA in some species of solanaceae. Journal of Phytopathology (Berlin) 144(3): 163-165, 1996

Tomato golden mosaic virus leftward gene expression: autoregulation of geminivirus replication protein. Virology 195(1): 275-280, 1993

Development and genetic mapping of a SCAR marker for the bean golden yellow mosaic geminivirus resistance gene BGM-1. Annual report 50(50): 77-78, 2007