EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Purification and biochemical characterization of 1-aminocyclopropane-1-carboxylate oxidase from banana fruit


Purification and biochemical characterization of 1-aminocyclopropane-1-carboxylate oxidase from banana fruit



Phytochemistry 39(1): 15-20



ISSN/ISBN: 0031-9422

A novel method using PEG and ammonium sulphate was used to concentrate ACC oxidase from a crude extract prepared from the pulp of ripe fruit of banana (Musa AAA group, Cavendish subgroup). The 145-fold purification of the ACC oxidase to electrophoretic homogeneity was carried out in four chromatographic steps: hydrophobic interaction, anion exchange, chromatofocusing and gel filtration. The molecular mass of the purified enzyme was estimated to be 40,000 by gel filtration and 36,000 by SDS-PAGE, indicating that the enzyme is active as a monomer. The enzyme was recognized by a polyclonal antibody directed against a recombinant polypeptide derived from the tomato ACC oxidase, showing that the banana enzyme shares immunogenic epitopes with ACC oxidases from other fruits. An isoelectric point at pH 4.9 was estimated by chromatofocusing. The K-m with respect to ACC was found to be 56 mu-M. Ascorbate, Fe-2+ and sodium bicarbonate were required for the activity of the enzyme, and the presence of ascorbate was found to be necessary to avoid loss of activity during the purification. In general, the properties of the ACC oxidase from this monocotyledonous source resemble those of the enzyme previously purified from apple fruit.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 002680616

Download citation: RISBibTeXText

Related references

Purification and biochemical characterization of 1-aminocyclopropane-1-carylate oxidase from banana fruit. Phytochemistry 39(1): 15-20, 1995

Purification and characterization of 1-aminocyclopropane-1-carboxylate oxidase from apple fruit. Proceedings of the National Academy of Sciences of the United States of America 89(20): 9789-9793, 1992

Purification and molecular characterization of 1 aminocyclopropane 1 carboxylate acc oxidase from ripening apple fruit. Plant Physiology 99(1 Suppl): 22, 1992

Expression of 1-aminocyclopropane-1-carboxylate synthase and 1-aminocyclopropane-1-carboxylate oxidase genes during ripening in 'Rendaiji' persimmon fruit. Journal of the Japanese Society for Horticultural Science 75(2): 178-184, 2006

Differential expression and internal feedback regulation of 1-aminocyclopropane-1-carboxylate synthase, 1-aminocyclopropane-1-carboxylate oxidase, and ethylene receptor genes in tomato fruit during development and ripening. Plant physiology 118(4): 1295-1305, 1998

Characterization and expression analysis of a banana gene encoding 1-aminocyclopropane-1-carboxylate oxidase. Biochemistry and molecular biology international 41(5): 941-950, 1997

CRISPR/Cas9-mediated genome editing of MaACO1 (aminocyclopropane-1-carboxylate oxidase 1) promotes the shelf life of banana fruit. Plant Biotechnology Journal 19(4): 654-656, 2021

Molecular cloning and characterization of a novel 1-aminocyclopropane-1-carboxylate oxidase gene involved in ripening of banana fruits. Journal of Agricultural and Food Chemistry 53(21): 8239-8247, 2005

Expression of 1-aminocyclopropane-1-carboxylate synthase and 1-aminocyclopropane-1-carboxylate oxidase genes during ripening in 'Rendaiji' persimmon fruit. Journal of the Japanese Society for Horticultural Science 75(2): 178-184, 2006

Cloning and characterization of a cDNA for 1-aminocyclopropane-1-carboxylate oxidase from papaya fruit. Journal of agricultural and food chemistry 45(2): 526-530, 1997

Characterization of 1-aminocyclopropane-1-carboxylate oxidase partially purified from cherimoya fruit. Journal of Agricultural and Food Chemistry 44(3): 730-735, 1996

Biochemical characterization of 1-aminocyclopropane-1-carboxylate oxidase in mung bean hypocotyls. Journal of Biochemistry and Molecular Biology 31(1): 70-76, 1998

Expression, purification and characterization of 1-aminocyclopropane-1-carboxylate oxidase from tomato in Escherichia coli. Biochemical Journal 307: 77-85, 1995

Partial characterization of 1-aminocyclopropane-1-carboxylate oxidase from excised mesocarp tissue of winter squash fruit. Scientific Reports of the Faculty of Agriculture, Okayama University 82: 49-59, 1993

Extraction and properties of 1-aminocyclopropane-1-carboxylate synthase in banana fruit. Journal of the Japanese Society for Horticultural Science 69(6): 696-701, 2000