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Restriction analysis of the mitochondrial DNA of male-sterile and maintainer lines of pearl millet, Pennisetum americanum L



Restriction analysis of the mitochondrial DNA of male-sterile and maintainer lines of pearl millet, Pennisetum americanum L



Plant Breeding 114(3): 256-258



Mitochondrial DNA from two pairs of cytoplasmic male-sterile (cms) and maintainer lines of pearl millet was investigated by restriction-enzyme analysis and Southern-blot hybridization using three mitochondrial gene probes. Each pair of male-sterile and maintainer lines was of a different nuclear origin. The objective was to distinguish differences in the DNA base-sequence organization of the mitochondrial genomes of cms and maintainer lines from the two sources. Restriction-enzyme analysis revealed differences between the different cms and maintainer lines. Southern-blot hybridization experiments using cloned mitochondrial gene probes further distinguished differences between different lines. It is expected that the restriction-fragment-length polymorphisms revealed in the Southern-blot-hybridization experiments will be useful in distinguishing and classifying cms and maintainer lines obtained from different nuclear backgrounds.

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Accession: 002688315

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DOI: 10.1111/j.1439-0523.1995.tb00806.x


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