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The aspartate aminotransferase-P2 gene from Lupinus angustifolius

The aspartate aminotransferase-P2 gene from Lupinus angustifolius

Plant Physiology 106(4): 1683-1684

Nucleotide and deduced amino acid sequences are reported for the aspartate aminotransferase [D-alanine transaminase](AAT)-P1 gene (EMBL/GenBank/DDBJ accession number L23875), isolated from a genomic library of cv. Uniharvest. It is 5990 bp long, containing 1961 bp of 5'-flanking sequence and 375 bp of 3'-untranslated region. The coding region comprises 12 exons. The transcription start site is located at position 1962, a putative TATA box at position 1928 and poly(A) addition signals at positions 5632, 5688 and 5814. An AAAGAT motif associated with nodule/root expression appears at positions 681 and 930, while a CTCTT nodule-specific motif occurs at positions 204, 1157 and 1484. Two sequences associated with light regulation of gene expression, GATAAG and GGTTAC, are located at positions 527 and 413, respectively. The putative polypeptide encoded by the ORF is 420 amino acids long and has a MW of 45 781 Da. No similarity in the intron-exon borders was evident between this AAT-P1 sequence and animal cytosolic genes. L. angustifolius AAT-P1 is expressed in roots, root nodules, seeds and leaves. Genomic Southern hybridization data are consistent with a single copy of the AAT-P1 gene in the L. angustifolius genome.

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Accession: 002709959

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PMID: 7846167

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