+ Translate

The aspartate aminotransferase-P2 gene from Lupinus angustifolius

, : The aspartate aminotransferase-P2 gene from Lupinus angustifolius. Plant Physiology 106(4): 1683-1684

Nucleotide and deduced amino acid sequences are reported for the aspartate aminotransferase [D-alanine transaminase](AAT)-P1 gene (EMBL/GenBank/DDBJ accession number L23875), isolated from a genomic library of cv. Uniharvest. It is 5990 bp long, containing 1961 bp of 5'-flanking sequence and 375 bp of 3'-untranslated region. The coding region comprises 12 exons. The transcription start site is located at position 1962, a putative TATA box at position 1928 and poly(A) addition signals at positions 5632, 5688 and 5814. An AAAGAT motif associated with nodule/root expression appears at positions 681 and 930, while a CTCTT nodule-specific motif occurs at positions 204, 1157 and 1484. Two sequences associated with light regulation of gene expression, GATAAG and GGTTAC, are located at positions 527 and 413, respectively. The putative polypeptide encoded by the ORF is 420 amino acids long and has a MW of 45 781 Da. No similarity in the intron-exon borders was evident between this AAT-P1 sequence and animal cytosolic genes. L. angustifolius AAT-P1 is expressed in roots, root nodules, seeds and leaves. Genomic Southern hybridization data are consistent with a single copy of the AAT-P1 gene in the L. angustifolius genome.

(PDF 0-2 workdays service)

Accession: 002709959

PMID: 7846167

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Kirk, C.A.; Mett, V.; Reynolds, P.H., 1994: The aspartate aminotransferase-P1 gene from Lupinus angustifolius. Nucleotide and deduced amino acid sequences are reported for the aspartate aminotransferase [D-alanine transaminase](AAT)-P1 gene (EMBL/GenBank/DDBJ accession number L23875), isolated from a genomic library of cv. Uniharvest. It is 5990 bp long, c...

Winefield, C.S.; Reddington, B.D.; Jones, W.T.; Reynolds, P.H.; Farnden, K.J., 1994: Cloning and characterization of a cDNA encoding aspartate aminotransferase-P1 from Lupinus angustifolius root tips. A root tip cDNA library, constructed in the lambda Zap II expression vector, was immunoscreened with a monoclonal antibody raised against aspartate aminotransferase-P1 from Lupinus angustifolius L. var Uniharvest. One 1452-base pair clone was isol...

Pakendorf, Kw, 1970: Studies on the use of mutagenic agents in Lupinus. I. The effect of gamma-ray dose on plant survival in Lupinus angustifolius, Lupinus albus, Lupinus luteus and Lupinus mutabilis

Subramania Sastry, M.R.; Velayudhan Nayar, C.K.; Bamaswamy, B.; Venkatachalam, K., 1942: Studies on Lupinus angustifolius L. 1. A preliminary investigation on the use of the seeds of Lupinus angustifolius as cattle food. The alkaloid content of the seeds was found to be 0.13 per cent. Debittered seeds fed to a goat did not produce any symptom of poisoning. The seeds may with safety be fed to bullocks at a rate of 3 lb. per day.

Aniszewski, T.; Drozdov, S.N.; Kholoptseva, E.S.; Kurets, V.K.; Obshatko, L.A.; Popov, E.G.; Talanov, A.V., 2001: Effects of light and temperature parameters on net photosynthetic carbon dioxide fixation by whole plants of five lupin species (Lupinus albus L., Lupinus angustifolius L., Lupinus luteus L., Lupinus mutabilis). Net photosynthetic CO< sub>2</ sub> uptake by whole plants of five lupin species (Lupinus albus, L. angustifolius, L. luteus, L. mutabilis and L. polyphyllus) was measured and modelled in laboratory conditions by an infrared gas analys...

Kordan, B.; Ciepielewska, D., 2002: Aphids Aphididae on narrow-leaf lupine Lupinus angustifolius L Mszyce Aphididae lubinu waskolistnego Lupinus angustifolius L. The purpose of the study was to determine the species composition and number of aphid fauna on some species of narrow-leaf lupine (Lupinus angustifolius L.). It was found that both Acyrthosiphon pisuni and Aphis fabae were the dominant species on...

Williams, W.H.rrison, J., 1983: Alkaloid concentration during development in three Lupinus species and the expression of genes for alkaloid biosynthesis in seedlings Lupinus albus, Lupinus angustifolius, Lupinus metabilis, lupine. Phytochemistry 2(1): 85-90

Nadolska Orczyk A., 1992: Somatic embryogenesis of agriculturally important lupin species lupinus angustifolius lupinus albus lupinus mutabilis. Somatic embryos were obtained from immature cotyledons of Lupinus angustifolius, L. albus and L. mutabilis but not from L. luteus. Different kinds of basal media and plant growth regulators in primary and secondary culture were tested. The best in...

Przybylska, J., 1964: Contents of crude protein and some essential amino acids in hay of Lupinus luteus, Lupinus angustifolius, and Lupinus albus. Comparisons were made of two cultivars of each species in respect of aminoacid composition and five cultivars in respect of protein content. The content of crude protein was highest in Lupinus angustifolius 'Obornicki', with 22.7%. Littl...

Egle, K.; Soliman, M.F.; Romer, W.; Gerke, J., 2001: Effect of citrate on the uptake of copper and cadmium by Lupinus albus, Lupinus luteus and Lupinus angustifolius. During P deficiency, several lupin species show an increased efflux of organic acid anions, which increases the solubility of P and metals such as Fe, Al, Zn, Cu and Cd. The effect of these organic acid anions on the uptake of Cu and Cd is not wel...