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A TaqI restriction fragment length polymorphism in the bovine calpastatin gene



A TaqI restriction fragment length polymorphism in the bovine calpastatin gene



Journal of Animal Science 73(12): 3790



A cDNA clone obtained from cattle skeletal muscle by reverse transcriptase-polymerase chain reaction was used as a calpastatin probe. The probe was 425 bp in length and contained between 1059 and 1480 nucleotides, which code for regions II, III and IV of domain 2 and region I of domain 3, relative to pig calpastatin. Four bands were detected and the sizes of the bands were 14, 9, 8.5 and 5 kb.

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Accession: 002735184

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