+ Translate

A more rapid method of enzyme digestion and hypotonic treatment for plant chromosome specimens

, : A more rapid method of enzyme digestion and hypotonic treatment for plant chromosome specimens. Hereditas Beijing 17(5): 48

An improved method of preparing plant chromosome specimens is described involving fixing in methanol and glacial acetic acid for 2 h and treating with a mixture of cellulase and pectinase at 37 degrees C for 10 minutes.

(PDF 0-2 workdays service)

Accession: 002738797

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Chen R.; Song W.; L.X., 1982: Wall degradation hypotonic method of preparing chromosome samples in plant and its significance in the cyto genetics. The wall degradation hypotonic method of preparing mitotic chromosome samples in plants was studied. The chromosome count, karytoype analysis, Giemsa banding and SEM [scanning electron microscopy] observation of chromosomes obtained by testing 105...

Penna M.L.F.; Fontoura E.R.C.; Pena B.M.A.; Pena S.D.J., 1989: Chromosome digestion with the restriction enzyme alu i a simple method of c banding. Treatment of freshly prepared human chromosomes with low concentrations (10 U/ml) of the restriction enzyme Alu I, followed by Giemsa staining, consistently produces good-quality C-banding. This CAG (C-bands by Alu I using Giemsa) banding is simpl...

Pequerul, A.; Perez, C.; Madero, P.; Val, J.; Monge, E., 1993: A rapid wet digestion method for plant analysis. Fragoso, M A C [Editor], van Beusichem, M L [Editor] Developments in Plant and Soil Sciences; Optimization of plant nutrition 3-6

Kennedy J.F.; Noy R.J.; Stead J.A.; White C.A., 1989: A new rapid enzyme digestion method for predicting in vitro protein quality pdd index. The officially accepted Protein Efficiency Ratio (PER) assay has limited applicability as a quality control and process monitoring assay due to the 28 days required to complete the assay. The ultimate measure of protein nutritional quality is the...

Bullerdiek, J.; Bartnitzke, S.; Schloot, W., 1979: A rapid and simple sandwich-method used for chromosome analysis from small fetal and adult biopsy specimens. A new Sandwich-Method is described which uses aborted material and yields a high frequency of metaphases. Chromosome studies may be performed after 7--8 days without subculturing. It is possible to freeze the original tissue for later cell culture...

Beck A., 1987: A method for rapid extraction of providencia chromosomal dna and restriction enzyme digestion in agarose pellets. Letters In Applied Microbiology: 41-46

D.Z.euw, D.J.; Smith, N.A., 1963: A rapid method for display mounting of certain plant disease specimens. Mounting dried leaves by laminating them between sheets of flexible plastic such as Mylar (duPont) is described. The process has proven to be easy and relatively inexpensive. Some of the many potential uses of specimens laminated in this type of p...

Bald, Wb, 1975: A proposed method for specifying the temperature history of cells during the rapid cool-down of plant specimens. Journal of experimental botany: 26 (90) 103-119

van Erp, P.E.; Brons, P.P.; Boezeman, J.B.; de Jongh, G.J.; Bauer, F.W., 1988: A rapid flow cytometric method for bivariate bromodeoxyuridine/DNA analysis using simultaneous proteolytic enzyme digestion and acid denaturation. This report describes an immunocytochemical procedure for the simultaneous quantification of bromodeoxyuridine (BrdUrd) incorporated into cellular DNA and total DNA content in individual cells in suspension. Improvement of existing methods was ach...

Blanco, M., A.; Whitten, W.M.rk; Penneys, D., S.; Williams, N., H.; Neubig, K., M.; Endara, L., 2006: A simple and safe method for rapid drying of plant specimens using forced-air space heaters. We describe the use of forced-air space heaters for drying herbarium specimens with a setup that is simpler, lighter, safer, and more economical than most previously described portable dryers. We have tested this method during collecting trips in...