EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

A mouse model for improving cell survival of bisected cattle embryos



A mouse model for improving cell survival of bisected cattle embryos



Agricultural & Food Science in Finland 5(5): 529-534



Morula and blastocyst stage embryos recovered from B6D2F-1 mice were bisected with a metal microblade in M2 medium with or without sucrose and/or cytochalasin B supplementation. Cell lysis was determined by staining the embryos with Hoechst 33258 and propidium iodide. Lysed cells take up both stains but non-lysed cells only the Hoechst 33258 stain, resulting in pink fluorescence for lysed cells and blue fluorescence for non-lysed cells under UV excitation. During bisection of morulae, the presence of cytochalasin B decreased the proportion of lysed cells in both the absence (P=0.0001) and presence of sucrose (P=0.001). During bisection of blastocysts the average proportion of lysed cells was slightly lower in the presence of cytochalasin than that in the control medium, but the effect was not statistically significant (P=0.34). No effect of sucrose was observed in either demi-morulae or demi-blastocysts. These results are essentially similar to those obtained in simultaneous experiments with cattle embryos, suggesting that the simpler mouse model might be useful for developing less traumatic bisection protocols for cattle embryos.

(PDF 0-2 workdays service: $29.90)

Accession: 002738817

Download citation: RISBibTeXText



Related references

The survival of bisected cattle embryos without zonae pellucidae. Theriogenology 27(1): 257, 1987

Development of reconstituted mouse embryos produced from the cytoplast of bisected oocytes or pronuclear-stage embryos and single blastomeres of 2-cell stage embryos. Theriogenology 38(5): 921-934, 1992

Survival of bisected mouse embryos after exposure to pronase and medium free of calcium and magnesium. Theriogenology 27(6): 859-868, 1987

Cell loss in bisected mouse, sheep and cow embryos. Theriogenology 32(1): 115-122, 1989

Cell allocation in twin half mouse embryos bisected at the 8-cell stage: implications for preimplantation diagnosis. Molecular Reproduction and Development 36(1): 16-22, 1993

The developmental potential of mouse demi-embryos bisected from frozen storage embryos by vitrification. Journal of Chinese Society of Animal Science 17(3-4): 21-28, 1988

Development of reconstituted mouse embryos produced from bisected and electrofused pronuclear-stage embryos. Theriogenology 37(3): 655-663, 1992

Transplantation of bisected cattle embryos. Medycyna Weterynaryjna 44(7): 412-414, 1988

Transfer of bisected cattle embryos within the same zona pellucida. Reproduction in Domestic Animals 26(2): 91-93, 1991

Factors affecting the survival of bisected sheep embryos in vivo. Theriogenology 36(3): 379-388, 1991

Factors affecting in vitro and in vivo viability of bisected cattle embryos. Theriogenology 37(1): 199, 1992

Freezing experiments with normal and bisected mouse embryos. Cryobiology 25(6): 0-568, 1988

The developmental potential of bisected frozen thawed mouse embryos. Japanese Journal of Animal Reproduction 32(3): 153-155, 1986

In vitro culture of isolated blastomeres and bisected embryos in mouse. Korean Journal of Animal Science 32(12): 781-784, 1990