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Antagonistic effect of bifidobacteria and lactoferrin on pathogenic Escherichia coli in simulated gut ecological system

Antagonistic effect of bifidobacteria and lactoferrin on pathogenic Escherichia coli in simulated gut ecological system

Indian Journal of Dairy Science 50(1): 50-55

A laboratory model biofermenter (Multigen, USA) was used for in vitro simulation of the intestinal ecological system to assess the combined effect of Bifidobacterium infantis and lactoferrin against enteropathogenic E. coli O111. Using full-term infant formula as substrate, live cells of B. infantis were added to the system to give 109 c.f.u. B. infantis/ ml, and, after a predetermined period, the system was challenged by addition of E. coli at 106 c.f.u./ml.

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