Application of in vivo fluorometry to determine soil mobility and soil adsorptivity of photosynthesis-inhibiting herbicides

Yanase, D.; Chiba, M.; Yagi, K.; Kawata, M.; Takagi, Y.

Meyer, M. T. , Thurman, E. M. Acs Symposium Series; Herbicide Metabolites In Surface Water And Groundwater: 77-84

1996


Accession: 002758199

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Abstract
Using chlorophyll a fluorescence as an indicator of photosynthesis inhibition, two bioassay systems were devised to determine soil mobility and soil adsorptivity of newly synthesized photosynthesis-inhibiting imidazolidine derivatives, OK-8901 and OK-9201. One rather primitive method, fluorometry using cucumber cotyledon discs, showed accuracy comparable to GC or HPLC in assessing leachability of the two compounds in a soil column assay. Another more efficient method utilizes a microplate scanner to measure fluorescence intensity in suspended microalgal cells (Chlorella vulgaris Beijer), and was used in determination of the soil adsorption coefficient (Koc). The results suggested that OK-9201 is highly soil-leachable, while OK-8901 showed intermediary soil adsorptivity between diuron and atrazine.