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bcl-2 acts early to restrict Semliki Forest virus replication and delays virus-induced programmed cell death


, : bcl-2 acts early to restrict Semliki Forest virus replication and delays virus-induced programmed cell death. Journal of Virology 71(2): 1583-1590

As characterized by morphological assessment and terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling, Semliki Forest virus (SFV) infection of rat prostatic adenocarcinoma cells triggers an apoptotic cell response. Cell death proceeded more rapidly following infection with the neurovirulent L10 strain of SFV than with the avirulent A7 strain. Overexpression of the antiapoptotic proto-oncogene bcl-2 allowed survival of cultures infected with either strain of virus. bcl-2 overexpression drastically reduced the numbers of productively infected cells within the cultures. In situ hybridization for viral message-sense RNA coupled with immunostaining for viral protein indicated that bcl-2 functions at an early stage of the virus life cycle, at entry, pretranscriptional events or at transcription, to inhibit virus replication. Double-immunofluorescent labeling for bcl-2 and viral glycoproteins revealed double-positive cells, demonstrating that with time, this early block in replication can be overcome. These productively infected bcl-2-expressing cells do, with time, undergo apoptosis. As a result of changing the balance between cell death and cell division by restricting productive virus replication and delaying virus-induced cell death, bcl-2 expression led to the establishment of chronically infected cell lines which could be passaged.


Accession: 002763184

PMID: 8995685

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Related references

Allsopp, T.E.; Shah, G.; Poirier, G.; Scallan, M.; Fazakerley, J.K., 1996: Semliki forest virus induced programmed cell death. Biochemical Society Transactions 24(4): 573S

van Steeg, H.; Thomas, A.; Verbeek, S.; Kasperaitis, M.; Voorma, H.O.; Benne, R., 1981: Shutoff of neuroblastoma cell protein synthesis by Semliki Forest virus: loss of ability of crude initiation factors to recognize early Semliki Forest virus and host mRNA's. A crude ribosomal wash containing the initiation factors of protein synthesis was isolated from mouse neuroblastoma cells 8 h after infection with Semliki Forest virus (SFV). The activity of this wash was compared with that of a wash from control...

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