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Bcl-2 protects mice against fatal alphavirus encephalitis

, : Bcl-2 protects mice against fatal alphavirus encephalitis. Proceedings of the National Academy of Sciences of the United States of America 93(10): 4810-4815

Virus-induced apoptosis has been well characterized in vitro, but the role of apoptosis in viral pathogenesis is not well understood. The suicide of a cell in response to viral infection is postulated to be an important host defense for the organism, leading to a reduction in its total viral burden. However, virus-induced death of nonregenerating cells in the central nervous system may be detrimental to the host. Therefore, to investigate the role of apoptosis in the pathogenesis of fatal encephalitis, we constructed a recombinant alphavirus chimera that expresses the antiapoptotic gene, bcl-2, in virally infected neural cells. Infection of neonatal mice with the alphavirus chimera expressing human bcl-2 (Sindbis virus (SIN)/bcl-2) resulted in a significantly lower mortality rate (7.5%) as compared with infection with control chimeric viruses containing a chloramphenicol acetyltransferase (CAT) reporter gene (SIN/CAT) (78.1%) or bcl-2 containing a premature stop codon (SIN/bcl-2stop) (72.1%) (P lt 0.001). Viral titers were reduced 5-fold 1 day after infection and 10-fold 6 days after infection in the brains of SIN/bcl-2-infected mice as compared to SIN/CAT or SIN/bcl2stop-infected mice. In situ end labeling to detect apoptotic nuclei demonstrated a reduction in the number of foci of apoptotic cells in the brains of mice infected with SIN/bcl-2 as compared with SIN/bcl-2stop. The reduction in apoptosis was associated with a reduction in the number of foci of cells expressing alphavirus RNA. Thus, the antiapoptotic gene, bcl-2, suppresses viral replication and protects against a lethal viral disease, suggesting an interaction between cellular genetic control of viral replication and cell death.

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Accession: 002763187

DOI: 10.2307/38858

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Schraufnagel, D.E.; Leech, J.A.; Pollak, B., 1986: Mycobacterium kansasii: colonization and disease. Mycobacterium kansasii has a wide range of virulence from fatal disease to infection and colonization. The laboratory report of growth of M. kansasii is often a problem for the clinician and an encumberance for the patient. To determine the differ...

Fathallah, D.M.; Zerria, K.; Barbouche, M.R.; Dellagi, K., 1999: A model to study the effects of a viral inactivator (beta-propiolactone) on DNA ligation and gene expression in E. coli and Cos cells. An experimental model to study the effects of viral inactivators on the biological properties of DNA was developed. Beta-propiolactone (betaPL) was used in this model and its effects on ligation, transfer and gene expression of naked DNA were asse...

Kuhlmann J.N.; Guerin Surville H.; Chretien Y., 1982: Vascularization of the os triquetrum and of the pisiform. The medial aspect of the [human] pisiform and the medial and dorsal aspects of the triquetrum are vascularized by the dorsal branch of the ulnar artery. The lateral aspect of the pisiform is vascularized by a pedicle which arises from the ulnar ar...

Brondz B.D.; Snegireva A.E.; Rassulin Y.A., 1971: Effect of enzymes on the interaction of immune lymphocytes with allogenic target cells. Problemy Gematologii i Perelivaniya Krovi 16(4): 11-17

Oken, M.M.; Marcus, P.M.; Hu, P.; Beck, T.M.; Hocking, W.; Kvale, P.A.; Cordes, J.; Riley, T.L.; Winslow, S.D.; Peace, S.; Levin, D.L.; Prorok, P.C.; Gohagan, J.K., 2005: Baseline chest radiograph for lung cancer detection in the randomized Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial. Background. The Prostate, Lung, Colorectal and Ovarian (PLCO) Cancer Screening Trial was initiated in 1992 to examine cause-specific mortality reduction from screening for these four cancers in men and women. We report lung cancer detection result...

Ajani, J.A., 1994: Contributions of chemotherapy in the treatment of carcinoma of the esophagus: results and commentary. Seminars in Oncology 21(4): 474-482

Wosylus, A.; Meier, K.; Prots, Y.; Schnelle, W.; Rosner, H.; Schwarz, U.; Grin, Y., 2010: Unusual silicon connectivities in the binary compounds GdSi5, CeSi5, and Ce2Si7. Angewandte Chemie 49(47): 9002-9006

SCHENK, V.W., 1963: Haemorrhages In Spinal Cord With Syringomyelia In A Patient With Haemophilia. Acta Neuropathologica 3: 306-308

Anonymous, 1985: Pulmonary fat accumulation after parenteral fat emulsion- Real or artifactual?. Recent reports indicate that pulmonary fat may accumulate in preterm infants who receive a fat emulsion during parenteral feeding. Autopsies of the lungs of preterm infants receiving such feeding revealed pulmonary fat accumulation, while those of...

Krol, G.; Sze, G.; Arbit, E.; Marcove, R.; Sundaresan, N., 1989: Intradural metastases of chordoma. Ajnr. American Journal of Neuroradiology 10(1): 193-195