EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Bean (Phaseolus vulgaris L.) protoplasts as a model system to study the expression and stability of recombinant seed proteins


, : Bean (Phaseolus vulgaris L.) protoplasts as a model system to study the expression and stability of recombinant seed proteins. Plant Cell Reports 16(10): 705-709

A suitable protocol for the transient expression of seed protein genes in protoplasts derived from cell suspension cultures of common bean has been established. Preliminary analyses of cultures to verify the synthesis of phaseolin--actively accumulated by the starting tissue, the developing cotyledon--showed that the protein was no longer synthesised after 5 days of culture. Transient expression of a phaseolin sequence, driven by a constitutive promoter, resulted in the accumulation of the correctly glycosylated and assembled protein. This system, when compared to tobacco protoplasts, largely avoids phaseolin fragmentation and the presence of contaminant polypeptides in the immunoprecipitates. Therefore, bean protoplasts are a good system to study the expression of wild-type as well as in-vitro-modified bean seed proteins.

Accession: 002763196

DOI: 10.1007/s002990050306

Download PDF Full Text: Bean (Phaseolus vulgaris L.) protoplasts as a model system to study the expression and stability of recombinant seed proteins


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Giovinazzo, G.G.eco, V.V.tale, A.B.llini, R., 1994: Protoplasts from Phaseolus vulgaris cell suspension cultures to study the expression and stability of recombinant seed proteins. Annual report7(37): 110

Raffi, S.A.; Newaz, M.A.; Khan, N., 2004: Stability analysis for pod and seed production in dry bean (Phaseolus vulgaris L.). Genotype (G) x environment (E) interaction was studied for number of pods and seeds per plant in 9 genotypes (PB-124, PB-125, PB-129, PB-142, PB-146, PB-S7, PB-S8, PB-S9 and PB-S10) of dry bean (P. vulgaris) under 4 environments during the rabi se...

Brown, J.W.S.; Osborn, T.C.; Bliss, F.A.; Hall, T.C., 1982: Bean lectins 1. relationships between agglutinating activity and electrophoretic variation in the lectin containing g 2 albumin seed proteins of french bean phaseolus vulgaris. Single seeds of over 100 bean cultivars were analyzed by 2-dimensional electrophoresis. The cultivars could be classified into 8 groups by virtue of their G2/albumin electrophoretic patterns. The polypeptide compositions of these types were largel...

Mutschler, M.A., 1980: The genetic control of globulin-1 seed protein, and its relationships to total protein content and quality in the dry bean (Phaseolus vulgaris L.) and male sterility in the dry bean (Phaseolus vulgaris L.). Most of this work has been summarized previously from other sources [see PBA 49, 7642; 50, 918, 5834 and 9753]. Inheritance of globulin-1 content was quantitative. Slight partial dominance was observed for high percentage protein and for high valu...

Brown, J.W.; Osborn, T.C.; Bliss, F.A.; Hall, T.C., 1982: Bean lectins : Part 1: Relationships between agglutinating activity and electrophoretic variation in the lectin-containing G2/albumin seed proteins of french bean (Phaseolus vulgaris L.). Single seeds of over 100 bean cultivars were analyzed by two-dimensional electrophoresis. The cultivars could be classified into eight groups by virtue of their G2/albumin electrophoretic patterns: TG2, SG2, VG2, PrG2, BG2, MG2, PG2, and PiG2, The...

Brown, J.W.S.; Osborn, T.C.; Bliss, F.A.; Hall, T.C., 1982: Bean lectins. Part 1: Relationships between agglutinating activity and electophoretic variation in the lectin-containing G2/albumin seed proteins of french bean (Phaseolus vulgaris L.). Theoretical and Applied Genetics 62(3): 263-271

Shri Dhar; Ram, H.H., 2002: Seed protein stability in French bean (Phaseolus vulgaris L.) under different fertility environments. Twenty eight French bean [Phaseolus vulgaris] cultivars including 9 prominent cultivars of pole, semi-pole and dwarf were evaluated under 4 fertility environments, E1 (0:0:40), E2 (60:60:40), E3 (120:60:40), and E4 (120:120:40 kg ha-1 N:P:K) for s...

Nimbalkar, C.A.; Baviskar, A.P.; Bajaj, V.H., 2006: AMMI approach for seed yield and stability of French bean (Phaseolus vulgaris L.). The performance of French bean (P. vulgaris) cultivars GRB-9810, Varun (C), GRB-9811, GRB-9701,GRB-9710, GRB 9801, GRB 9702, GRB-9812, HPR-35, Vaghya and HUR-137 was evaluated in a field experiment conducted in Pune, Maharashtra, India during the...

Gelin, J.P.; Forster, S.; Grafton, K.F.; Mcclean, P.E.; Kooas-Cifuentes, G.A., 2007: Analysis of seed zinc and other minerals in a recombinant inbred population of navy bean (Phaseolus vulgaris L.). Mineral deficiencies in common bean (Phaseolus vulgaris L.) negatively affect plant growth and development. Genetic differences in seed mineral concentrations have been detected among landraces and genotypes for trace elements such as Zn and Fe,...

Klein R.E.; Wyatt S.D.; Kaiser W.J.; Mink G.I., 1992: Comparative immunoassays of bean common mosaic virus in individual bean phaseolus vulgaris seed and bulked bean seed samples. Indirect enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody (MAb) or polyclonal antiserum (PC) and do immunoassay using a MAb were compared for the detection of bean common mosaic virus (BCMV) in seed of Phaseolus from the U.S....