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Benzoquinones of the beetles, Tribolium castaneum and Tribolium confusum

, : Benzoquinones of the beetles, Tribolium castaneum and Tribolium confusum. Preparative Biochemistry 25(3): 155-168

Tribolium castaneum and T. confusum were washed in HPLC-grade methanol, and the methanolic washes were analyzed by UV spectroscopy, reversed phase HPLC, and GC/MS. The methanolic washes from both species contained methyl-1,4-benzoquinone (MBQ) and ethyl-1,4-benzoquinone (EBQ). The amounts of MBQ recovered from the two species were not significantly different, but the amounts of EBQ and total benzoquinones (MBQ + EBQ) recovered from T. castaneum were significantly greater than for those recovered from T. confusum. The methods described are superior to previous methods for isolating, identifying, and quantifying the benzoquinones in these beetles, since they are relatively simple, fast, do not require handling of the beetles, and are sensitive enough to quantify the benzoquinones of a single beetle.

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Accession: 002763915

PMID: 8532638

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Related references

Pappas, P.W.; Wardrop, S.M., 1996: Quantification of benzoquinones in the flour beetles. Tribolium castaneum and Tribolium confusum. Benzoquinones were extracted from flour beetles by rinsing them for 8 min in methanol followed by homogenization in fresh methanol. This procedure recovered > 96% of the total benzoquinones from both species, with the methanolic rinse and super...

Wirtz, R.A.; Taylor, S.L.; Semey, H.G., 1978: Concentrations of substituted p-benzoquinones and 1-pentadecene in the flour beetles Tribolium confusum J. du Val and Tribolium castaneum (Herbst). Concentrations of 2-methyl-1,4-benzoquinone (MBQ), 2-ethyl-1,4-benzoquinone (EBQ) and 1-pentadecene (PD) in Tribolium confusum Duv. and T. castaneum (Hbst.) were found to be extremely low in newly emerged adults (<20 mu g/insect). The levels of...

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