+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Cantaloupe line CZW-30 containing coat protein genes of cucumber mosaic virus, zucchini yellow mosaic virus, and watermelon mosaic virus-2 is resistant to these three viruses in the field



Cantaloupe line CZW-30 containing coat protein genes of cucumber mosaic virus, zucchini yellow mosaic virus, and watermelon mosaic virus-2 is resistant to these three viruses in the field



Molecular breeding: new strategies in plant improvement(4): 279-290



Cantaloupe line CZW-30 containing coat protein gene constructs of cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic potyvirus (ZYMV), and watermelon mosaic virus 2 potyvirus (WMV-2) was investigated in the field over two consecutive years for resistance to infections by CMV, ZYMV, and/or WMV-2. Resistance was evaluated under high disease pressure achieved by mechanical inoculations and/or natural challenge inoculations by indigenous aphid vectors. Across five different trials, homozygous plants were highly resistant in that they never developed systemic symptoms as did the nontransformed plants but showed few symptomatic leaves confined close to the vine tips. Hemizygous plants exhibited a significant delay (2-3 weeks) in the onset of disease compared to control plants but had systemic symptoms 9-10 weeks after transplanting to the field. Importantly, ELISA data revealed that transgenic plants reduced the incidence of mixed infections. Only 8% of the homozygous and 33% of the hemizygous plants were infected by two or three viruses while 99% of the nontransformed plants were mixed infected. This performance is of epidemiological significance. In addition, control plants were severely stunted (44% reduction in shoot length) and had poor fruit yield (62% loss) compared to transgenic plants, and most of their fruits (60%) were unmarketable. Remarkably, hemizygous plants yielded 7.4 times more marketable fruits than control plants, thus suggesting a potential commercial performance. This is the first report on extensive field trials designed to assess the resistance to mixed infection by CMV, ZYMV, and WMV-2, and to evaluate the yield of commercial quality cantaloupes that are genetically engineered.

(PDF emailed within 0-6 h: $19.90)

Accession: 002770847

Download citation: RISBibTeXText

DOI: 10.1023/a:1009640229952


Related references

Field evaluation of transgenic squash containing single or multiple virus coat protein gene constructs for resistance to cucumber mosaic virus, watermelon mosaic virus 2, and zucchini yellow mosaic virus. Bio-Technology (New York) 13(13): 1458-1465, 1995

Resistance of transgenic hybrid squash ZW-20 expressing the coat protein genes of zucchini yellow mosaic virus and watermelon mosaic virus 2 to mixed infections by both potyviruses. Bio-Technology (New York) 13(13): 1466-1473, 1995

Effects and incidence of Cucumber mosaic virus, Watermelon mosaic virus and Zucchini yellow mosaic virus in New Zealand's only native cucurbit, Sicyos australis. Australasian Plant Pathology 35(1): 29-35, 2006

First Report of Zucchini yellow mosaic virus, Watermelon mosaic virus, and Cucumber mosaic virus in Bottlegourd (Lagenaria siceraria) in Serbia. Plant Disease 90(3): 380, 2019

First report of Zucchini yellow mosaic virus, Watermelon mosaic virus, and Cucumber mosaic virus in bottlegourd (Lagenaria siceraria) in Serbia. Plant Disease 90(3): 380, 2006

Watermelon mosaic virus-2, zucchini yellow mosaic virus and cucumber mosaic virus isolated from cucurbitaceous plants in Hokkaido. Research Bulletin of the Hokkaido National Agricultural Experiment Station (148): 65-73, 1987

Study of resistance to cucumber mosaic virus, watermelon mosaic virus and zucchini yellow mosaic virus in melon cultivars. Iran Agricultural Research 19(2): 129-144, 2000

Coat protein transgenic resistance to watermelon mosaic and zucchini yellows mosaic virus in squash and cantaloupe. Plant Disease 79(11): 1107-1109, 1995

Protection of transgenic plants expressing the coat protein gene of watermelon mosaic virus II or zucchini yellow mosaic virus against six potyviruses. Phytopathology 82(9): 940-946, 1992

Properties of viruses of the potyvirus group. 2. Buoyant density, S value particle morphology, and molecular weight of the coat protein subunit of bean yellow mosaic virus, pea mosaic virus, lettuce mosaic virus, and potato virus YN. Netherlands Journal of Plant Pathology 80(1): 19-27, 1974

Inheritance of resistance to watermelon mosaic virus in the cucumber line TMG-1: tissue-specific expression and relationship to zucchini yellow mosaic virus resistance. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 91(4): 699-706, 1995

Inheritance of resistance to the Moroccan watermelon mosaic virus in the cucumber line TMG-1 and its relationship to zucchini yellow mosaic virus resistance. Hortscience 31(4): 622, 1996

Inheritance of resistance to the Moroccan watermelon mosaic virus in the cucumber line TMG-1 and cosegregation with zucchini yellow mosaic virus resistance. Euphytica 95(2): 237-242, 1997

Detection of squash mosaic virus (SqMV), zucchini yellow mosaic virus (ZYMV) and cucumber mosaic virus (CMV) in melon seeds. Bulgarian Journal of Agricultural Science 11(1): 49-59, 2005

Comparative spatial spread overtime of Zucchini Yellow Mosaic Virus (ZYMV) and Watermelon Mosaic Virus (WMV) in fields of transgenic squash expressing the coat protein genes of ZYMV and WMV, and in fields of nontransgenic squash. Transgenic Research 15(5): 527-541, 2006