+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization and expression of a rice RAD23 gene

Characterization and expression of a rice RAD23 gene

Plant molecular biology 34(3): 557-562

In order to identify proteins that interact with plant transcriptional complexes, we performed a two-hybrid screen in yeast using a cDNA library from embryogenic rice suspension cultures and the plant transcriptional activator viviparous-1 (vp1) as 'bait'. In this screen, we detected an interaction between VP1 and a rice homologue of the Saccharomyces cerevisiae RAD23 gene (osRAD23). The RAD23 protein is associated with the general transcriptional machinery in yeast, and is believed to play a role in the processes of nucleotide excision repair in yeast and mammalian cells. This report is the first identification of a RAD23 homolog in plants. The osRAD23 amino acid sequence shares 50-60% similarity throughout its length with RAD23 sequences from yeast, mice, and man. osRAD23 contains a characteristic ubiquitin-like domain at its N-terminus, which is similar to other RAD23 genes. Analysis of the expressed sequence tag database identifies two different classes of RAD23 genes in both Arabidopsis and rice. Southern analysis of rice genomic DNA indicated the presence of at least two RAD23-like genes. A single transcript (1.5 kb) of osRAD23 was detected in total RNA from rice embryonic tissue, while three transcripts (1.8, 1.5 and 1.0 kb) were observed in total RNA from vegetative tissues of rice.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 002775009

Download citation: RISBibTeXText

PMID: 9225866

DOI: 10.1023/a:1005870227632

Related references

Identification and functional characterization of the Rad23 gene of the silkworm, Bombyx mori. Bioscience Reports 30(1): 19-26 2 P Following 26, 2009

Development of a microarray for two rice subspecies: characterization and validation of gene expression in rice tissues. Bmc Research Notes 7: 15, 2014

Molecular characterization and function analysis of a nucleotide excision repair gene Rad23 from Litopenaeus vannamei after Vibrio alginolyticus challenge. Fish and Shellfish Immunology 83: 190-204, 2018

Cloning, comparative mapping, and RNA expression of the mouse homologues of the Saccharomyces cerevisiae nucleotide excision repair gene RAD23. Genomics 31(1): 20-27, 1996

Two isoforms of plant RAD23 complement a UV-sensitive rad23 mutant in yeast. Plant Journal 13(6): 815-821, 1998

Constitutive expression of rice WRKY3 gene increases the endogenous jasmonic acid accumulation, PR gene expression and resistance to fungal pathogens in rice. 2012

Constitutive expression of rice WRKY30 gene increases the endogenous jasmonic acid accumulation, PR gene expression and resistance to fungal pathogens in rice. Planta 236(5): 1485-1498, 2012

Cloning and characterization of the granule-bound starch synthase II gene in rice: gene expression is regulated by the nitrogen level, sugar and circadian rhythm. Planta 218(2): 261-268, 2003

Expression and characterization of rice putative PAUSED gene. Acta Biochimica et Biophysica Sinica 40(10): 893-900, 2008

Characterization of a rice pollen-specific gene and its expression. American Journal of Botany 81(5): 552-561, 1994

Expression and characterization of a novel vacuole Na+/H+ antiporter gene in rice (Oryza sativa). Acta Agronomica Sinica 33(2): 327-332, 2007

Characterization of the Ferredoxin-Gogat gene (OsGog2 clone) expression in rice. Biologia Plantarum 50(2): 187-192, 2006

Characterization and transcriptional expression of the alpha-expansin gene family in rice. Molecules and Cells 20(2): 210-218, 2005

Structural characterization and expression pattern analysis of the rice PLT gene family. Acta Biochimica et Biophysica Sinica 43(9): 688-697, 2011

Characterization of the phosphofructokinase gene family in rice and its expression under oxygen deficiency stress. Frontiers in Plant Science 4: 125, 2013