+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization and quantitation of the apoproteins of high-density lipoprotein by capillary electrophoresis

Characterization and quantitation of the apoproteins of high-density lipoprotein by capillary electrophoresis

Analytical Biochemistry 243(1): 100-109

A method has been developed using capillary electrophoresis (CE) to quantitate plasma levels of apoprotein A-I (apoA-I) and apoprotein A-II (apoA-II) in high-density lipoprotein (HDL) samples. ApoA-I and apoA-II are resolved by CE in delipidated and non-delipidated HDL samples. Concentrations of apoA-I and apoA-II were calculated from their peak areas in the electropherogram. Results of the analysis of Sigma plasma standards (Controls 1 and 2) using CE are in good agreement with values obtained by Sigma using immunoturbidimetric assay. CE and reverse-phase high-performance liquid chromatography (RP-HPLC) were found to be complementary in the study of apoA-I and apoA-II. RP-HPLC resolves the isoforms of purified apoA-I and apoA-II, but it cannot resolve mixtures of them because the retention times of the isoforms overlap. CE separates apoA-I from apoA-II, but it does not resolve the isoforms. Matrix-assisted laser desorption/ ionization mass spectrometry was used to identify the isoforms of apoA-I and apoA-II by their molecular weight (M(r)) in fractions collected from RP-HPLC.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 002775080

Download citation: RISBibTeXText

PMID: 8954531

DOI: 10.1006/abio.1996.0487

Related references

High-density lipoprotein has different binding capacity for different apoproteins. The amyloidogenic apoproteins are easier to displace from high-density lipoprotein. Scandinavian Journal of Immunology 28(6): 653-658, 1988

Characterization of in vitro modified human high-density lipoprotein particles and phospholipids by capillary zone electrophoresis and LC ESI-MS. Journal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences 877(29): 3495-3505, 2009

Tetranitromethane modification of human high density lipoprotein (HDL3): inactivation of high density lipoprotein binding is not related to cross-linking of phospholipids to apoproteins. Journal of Lipid Research 28(3): 332-337, 1987

Analysis of high density lipoprotein apolipoproteins by capillary zone and capillary SDS gel electrophoresis. Journal of Lipid Research 39(1): 218-227, 1998

Isolation and characterization of high density lipoprotein apoproteins in the non-human primate (vervet). Journal of Biological Chemistry 254(14): 6716-6723, 1979

Isolation, characterization and metabolism of high density lipoprotein (HDL) apoproteins of the nonhuman primate (vervet). Dissertation Abstracts International B Sciences and Engineering, 409: 4089, 1980

Relationship of the parameters of body cholesterol metabolism with plasma levels of high density lipoprotein cholesterol and the major high density lipoprotein apoproteins. Journal of Lipid Research 26(9): 1079-1088, 1985

Direct quantitation of serum high density lipoprotein subfractions separated by gradient gel electrophoresis. Clinica Chimica Acta 172(2-3): 183-190, 1988

High-density lipoprotein cholesterol, non-high-density lipoprotein cholesterol, apoproteins and a case of a drug trial that did not fulfil expectations. Current Opinion in Lipidology 18(5): 603-606, 2007

Characterization of in vitro modified human very low-density lipoprotein particles and phospholipids by capillary electrophoresis. International Journal of Molecular Sciences 13(12): 16400-16417, 2012

Determination of the respective density distributions of low density and high density lipoprotein particles in bovine plasma and lymph by immunoassay of apoproteins a i and b. Reproduction Nutrition Development Suppl. 6(2): 227S, 1990

Cross-linking of apoproteins in high density lipoprotein by dimethylsuberimidate inhibits specific lipoprotein binding to membranes. Journal of Lipid Research 29(3): 319-324, 1988

High density lipoprotein and metabolic risk factors of ischemic heart disease in men aged up to 50 years part i. high density lipoprotein cholesterol high density lipoprotein triglycerides high density lipoprotein phospholipids and phospholipid fractions in dyslipidemias. Zywienie Czlowieka I Metabolizm 13(1): 26-37, 1986

High resolution nanotechnique for separation characterization and quantitation of micromolecules and macromolecules capillary electrophoresis. Horvath, C And J G Nikelly (Ed ) Acs (American Chemical Society) Symposium Series, 434 Analytical Biotechnology: Capillary Electrophoresis And Chromatography; 196th National Meeting Of The American Chemical Society, Los Angeles, California, Usa, September 25-30, 1988 X+213p American Chemical Society: Washington, D C , Usa Illus 1-35, 1990